CAJ | 학술논문

目的：观察不同浓度重组高迁移率族蛋白1（recombination high mobility group box protein 1，rHMGB1）对DEN2感染鼠源单核巨噬细胞Ana－1分泌TNF－α、NO及DEN复制的影响。方法：直接免疫荧光法鉴定DEN2吸附Ana－1细胞；qRT－PCR检测不同时间胞内病毒载量变化；双抗体夹心ELISA法检测感染不同时间TNF－α的分泌水平，Griess试剂检测细胞释放NO变化。结果：不同剂量rHMGB1处理Ana－1细胞后，对DEN2感染细胞的病毒抑制率（％）分别为41．53±2．12﹑55．30±1．59﹑74．75±1．12﹑86．35±1．42，差异有统计学意义（P＜0．05）。 rHMGB1处理后，感染细胞分泌TNF－α和NO水平均有所下降，差异有统计学意义（P＜0．05）。结论：rHMGB1可有效调节DEN2感染Ana－1细胞的TNF－α和NO分泌，并抑制DEN2的增殖。
목적：관찰불동농도중조고천이솔족단백1（recombination high mobility group box protein 1，rHMGB1）대DEN2감염서원단핵거서세포Ana－1분비TNF－α、NO급DEN복제적영향。방법：직접면역형광법감정DEN2흡부Ana－1세포；qRT－PCR검측불동시간포내병독재량변화；쌍항체협심ELISA법검측감염불동시간TNF－α적분비수평，Griess시제검측세포석방NO변화。결과：불동제량rHMGB1처리Ana－1세포후，대DEN2감염세포적병독억제솔（％）분별위41．53±2．12﹑55．30±1．59﹑74．75±1．12﹑86．35±1．42，차이유통계학의의（P＜0．05）。 rHMGB1처리후，감염세포분비TNF－α화NO수평균유소하강，차이유통계학의의（P＜0．05）。결론：rHMGB1가유효조절DEN2감염Ana－1세포적TNF－α화NO분비，병억제DEN2적증식。
Objective:To observe the effect of different concentration HMGB1 on the secretion of TNF-αand NO from Ana-1 infected with DEN2 and virus copy.Methods:DEN2 were proliferated and identified by conventional methods.The adherence of DEN2 to Ana-1 was observed by direct immunofluorescence and RT-PCR.The level of virus mRNA were detected by qRT-PCR.The concentration of TNF-αwas detected by ELISA.The concentration of NO was detected with Griess reagent.Results:Ana-1 was able to adhered for DEN2.Compared with DEN group,the inhibition ratio(%) of the level of virus mRNA in D-HMGB1-1 group,D-HMGB1-10 group,D-HMGB1-100 group,D-HMGB1-1000 group was 41.53 ±2.12,55.30 ±1.59,74.75 ±1.12,86.35 ±1.42.Compared with DEN group,the level of TNF-αand NO decreased in D-HMGB1 groups(P<0.05).Conclusion:HMGB1 can be effectively regulated of Ana-1 secreted inflammation factor of infected with DEN2,and inhibited DEN2 replication.