CAJ | 학술논문

病毒病是制约马铃薯产量和质量的重要因素。马铃薯脱毒试管苗、微型薯是生产脱毒种薯的重要环节。本研究于2010~2013年对收集自云南省的试管苗274个、微型薯356个，共计630个样品。应用电子显微镜观察，并采用DAS-ELISA检测了8种病毒：马铃薯S病毒、马铃薯卷叶病毒、马铃薯Y病毒、马铃薯X病毒、马铃薯A病毒、马铃薯M病毒、番茄斑萎病毒和烟草环斑病毒，发现试管苗和微型薯中马铃薯S病毒检出率最高（21.27%），其次是PLRV(5.71%)；还检测到了新出现的侵染马铃薯的番茄斑萎病毒和烟草环斑病毒。研究结果为马铃薯种薯生产过程中病毒病的检测防控提供了参考。
병독병시제약마령서산량화질량적중요인소。마령서탈독시관묘、미형서시생산탈독충서적중요배절。본연구우2010~2013년대수집자운남성적시관묘274개、미형서356개，공계630개양품。응용전자현미경관찰，병채용DAS-ELISA검측료8충병독：마령서S병독、마령서권협병독、마령서Y병독、마령서X병독、마령서A병독、마령서M병독、번가반위병독화연초배반병독，발현시관묘화미형서중마령서S병독검출솔최고（21.27%），기차시PLRV(5.71%)；환검측도료신출현적침염마령서적번가반위병독화연초배반병독。연구결과위마령서충서생산과정중병독병적검측방공제공료삼고。
The viral diseases are important constrains to yield and quality of potato. In vitro plantlets and minitubers are the key steps in the production of virus-free seed potato. In this research, 630 samples including 274 in vitro plantlets and 356 minitubers were col ected from 2010 to 2013 in Yunnan Province. The samples were detected through the electron microscopy. DAS-ELISA was used to test PVS, PLRV, PVY, PVX, PVA, PVM, TSWV and TRSV. The results showed that PVS and PLRV were two predominant viruses, and 21.27%and 5.71%of the samples were infected by PVS and PLRV, respectively. TSWV and TRSV were two emerging viruses infecting potato in China. The results could provide a consult for detection and control of potato virus diseases in potato production.