中国马铃薯
中國馬鈴藷
중국마령서
CHINESE POTATO
2015年
1期
33-36
,共4页
马铃薯黑痣病%菌丝%生物学特性
馬鈴藷黑痣病%菌絲%生物學特性
마령서흑지병%균사%생물학특성
potato black scurf%mycelium%biological property
马铃薯黑痣病是土传真菌性病害,在定西市马铃薯种植区均有发生,目前已成为影响马铃薯产量和品质的主要因素之一。本研究对马铃薯黑痣病菌从温度、光照、碳源、氮源以及pH方面进行了生物学特性的测定。结果表明:该病原菌菌丝在无光25℃条件下生长最快,在无光35℃条件下生长最慢;室温条件下培养1 d后用紫外线照射处理2h,然后室温持续光照培养4d的菌丝生长速率最大,持续黑暗培养4d的菌丝生长速率最慢;不同碳氮源对该菌菌丝生长均有影响,碳源为淀粉的培养基上菌丝生长最快、氮源为尿素的培养基上菌丝生长最快;培养基pH中性时菌丝生长速率最大。
馬鈴藷黑痣病是土傳真菌性病害,在定西市馬鈴藷種植區均有髮生,目前已成為影響馬鈴藷產量和品質的主要因素之一。本研究對馬鈴藷黑痣病菌從溫度、光照、碳源、氮源以及pH方麵進行瞭生物學特性的測定。結果錶明:該病原菌菌絲在無光25℃條件下生長最快,在無光35℃條件下生長最慢;室溫條件下培養1 d後用紫外線照射處理2h,然後室溫持續光照培養4d的菌絲生長速率最大,持續黑暗培養4d的菌絲生長速率最慢;不同碳氮源對該菌菌絲生長均有影響,碳源為澱粉的培養基上菌絲生長最快、氮源為尿素的培養基上菌絲生長最快;培養基pH中性時菌絲生長速率最大。
마령서흑지병시토전진균성병해,재정서시마령서충식구균유발생,목전이성위영향마령서산량화품질적주요인소지일。본연구대마령서흑지병균종온도、광조、탄원、담원이급pH방면진행료생물학특성적측정。결과표명:해병원균균사재무광25℃조건하생장최쾌,재무광35℃조건하생장최만;실온조건하배양1 d후용자외선조사처리2h,연후실온지속광조배양4d적균사생장속솔최대,지속흑암배양4d적균사생장속솔최만;불동탄담원대해균균사생장균유영향,탄원위정분적배양기상균사생장최쾌、담원위뇨소적배양기상균사생장최쾌;배양기pH중성시균사생장속솔최대。
The potato black scurf is a soil-borne fungal disease, which happens in al of the areas of Dingxi. It is one of the main factors influencing potato quantity and quality. This research was to characterize biological properties of the pathogen causing potato black scurf in terms of temperature, il umination, carbon source, nitrogen source and pH value. The mycelium grew the fastest at 25℃ and the slowest at 35℃ under dark conditions. When cultured for 1 d at 25℃ , treated with UV for 2 h, and then kept under light for 4 d at room temperature, the mycelium grew the fastest, while the mycelium grew the slowest when kept under darkness for 4 d. The mycelium growth was affected by different sources of carbon and nitrogen. Starch was the best carbon source and urea was the best nitrogen source for the mycelium growth. The mycelium grew fastest when pH was set at 7.0.