中国马铃薯
中國馬鈴藷
중국마령서
CHINESE POTATO
2015年
1期
19-33
,共15页
高云飞%闵凡祥%王文重%杨帅%魏琪%吕典秋%郭梅%吕涛
高雲飛%閔凡祥%王文重%楊帥%魏琪%呂典鞦%郭梅%呂濤
고운비%민범상%왕문중%양수%위기%려전추%곽매%려도
马铃薯%ITS%PCR%接骨木镰刀菌%分子检测
馬鈴藷%ITS%PCR%接骨木鐮刀菌%分子檢測
마령서%ITS%PCR%접골목렴도균%분자검측
potato%ITS%PCR%F. sambucinum%molecular detection
接骨木镰刀菌是马铃薯干腐病的主要致病菌，给黑龙江省马铃薯造成了一定的经济损失。采用马铃薯干腐病引物LDJ1对马铃薯干腐病、马铃薯晚疫病、马铃薯早疫病、马铃薯黑痣病的致病菌的ITS序列进行测定，只有马铃薯干腐病菌扩增出560 bp特异性片段。再分别对接骨木镰刀菌、燕麦镰刀菌和茄病镰孢变种的ITS序列进行BLAST比对分析，针对接骨木镰刀菌设计了特异引物L2，扩增特异性产物为278 bp，该引物所建立的PCR检测体系可检测DNA含量在50 pg/μL以上的目的基因。
接骨木鐮刀菌是馬鈴藷榦腐病的主要緻病菌，給黑龍江省馬鈴藷造成瞭一定的經濟損失。採用馬鈴藷榦腐病引物LDJ1對馬鈴藷榦腐病、馬鈴藷晚疫病、馬鈴藷早疫病、馬鈴藷黑痣病的緻病菌的ITS序列進行測定，隻有馬鈴藷榦腐病菌擴增齣560 bp特異性片段。再分彆對接骨木鐮刀菌、燕麥鐮刀菌和茄病鐮孢變種的ITS序列進行BLAST比對分析，針對接骨木鐮刀菌設計瞭特異引物L2，擴增特異性產物為278 bp，該引物所建立的PCR檢測體繫可檢測DNA含量在50 pg/μL以上的目的基因。
접골목렴도균시마령서간부병적주요치병균，급흑룡강성마령서조성료일정적경제손실。채용마령서간부병인물LDJ1대마령서간부병、마령서만역병、마령서조역병、마령서흑지병적치병균적ITS서렬진행측정，지유마령서간부병균확증출560 bp특이성편단。재분별대접골목렴도균、연맥렴도균화가병렴포변충적ITS서렬진행BLAST비대분석，침대접골목렴도균설계료특이인물L2，확증특이성산물위278 bp，해인물소건립적PCR검측체계가검측DNA함량재50 pg/μL이상적목적기인。
F. sambucinum is the main pathogenic bacterium bringing about potato dry rot and causes economic losses to some extent in Heilongjiang Province. Tests were made to the ITS sequence of Fusarium spp., Phytophthora infestans, Alternaria solani and Rhizoctonia solani using the primer LDJ1, and 560 bp specific fragment was amplified only from Fusarium spp.. Comparative analysis of BLAST was conducted on ITS sequence of F. sambucinum, F. avenaceum, and F. solani var. coeruleum, and the specific primer L2 was designed. The target band amplified was 278 bp. The sensitivity of PCR detection system based on this primer was 50 pg/μL for the target gene.
