中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
1期
97-102
,共6页
张桢%王青青%张根花%刘琴
張楨%王青青%張根花%劉琴
장정%왕청청%장근화%류금
结肠癌%Musashi 1%CD105%微血管密度
結腸癌%Musashi 1%CD105%微血管密度
결장암%Musashi 1%CD105%미혈관밀도
Colon cancer%Musashi 1%CD105%Microvessel density
目的：探讨Musashi 1在结肠癌中的表达与肿瘤微血管密度（ MVD）及预后的关系。方法：应用实时荧光定量PCR的方法检测36对结肠癌组织及其配对的正常结肠组织中Musashi 1 mRNA的表达情况。通过免疫组化法对96例结肠癌组织中Musashi 1表达情况和微血管密度（ MVD）进行检测。结合随访术后生存情况，统计分析Musashi 1表达与结肠癌病理参数、预后及MVD之间的相关性。结果：实时荧光定量PCR法结果显示，在结肠癌组织中，Musashi 1 mRNA的相对表达水平显著高于正常结肠组织（P＜0．05）。免疫组化结果显示，96例结肠癌组织中，有61例（63．5％）高表达Musashi 1。 Musashi 1的表达与患者性别、肿瘤大小、分化程度无相关性（P＞0．05），而与肿瘤的浸润深度、Dukes分期、淋巴转移、TNM分期密切相关（ P＜0．05）。 Kaplan－Meier生存分析显示，Musashi 1高表达患者的5年生存率显著低于Musashi 1低表达者（23．0％vs 60．0％；P＜0．01），且Musashi 1的表达与结肠癌血管密度相关（P＜0．01）。结论：结肠癌中Musashi 1的表达较正常癌旁组织高，具有统计学意义。结肠癌中Musashi 1的表达与肿瘤浸润深度、Dukes分期、淋巴转移、TNM分期和血管密度密切相关。 Musashi 1蛋白可通过促进瘤内血管增生，从而促进结肠癌的生长和侵袭转移，进一步影响结肠癌患者的预后。
目的：探討Musashi 1在結腸癌中的錶達與腫瘤微血管密度（ MVD）及預後的關繫。方法：應用實時熒光定量PCR的方法檢測36對結腸癌組織及其配對的正常結腸組織中Musashi 1 mRNA的錶達情況。通過免疫組化法對96例結腸癌組織中Musashi 1錶達情況和微血管密度（ MVD）進行檢測。結閤隨訪術後生存情況，統計分析Musashi 1錶達與結腸癌病理參數、預後及MVD之間的相關性。結果：實時熒光定量PCR法結果顯示，在結腸癌組織中，Musashi 1 mRNA的相對錶達水平顯著高于正常結腸組織（P＜0．05）。免疫組化結果顯示，96例結腸癌組織中，有61例（63．5％）高錶達Musashi 1。 Musashi 1的錶達與患者性彆、腫瘤大小、分化程度無相關性（P＞0．05），而與腫瘤的浸潤深度、Dukes分期、淋巴轉移、TNM分期密切相關（ P＜0．05）。 Kaplan－Meier生存分析顯示，Musashi 1高錶達患者的5年生存率顯著低于Musashi 1低錶達者（23．0％vs 60．0％；P＜0．01），且Musashi 1的錶達與結腸癌血管密度相關（P＜0．01）。結論：結腸癌中Musashi 1的錶達較正常癌徬組織高，具有統計學意義。結腸癌中Musashi 1的錶達與腫瘤浸潤深度、Dukes分期、淋巴轉移、TNM分期和血管密度密切相關。 Musashi 1蛋白可通過促進瘤內血管增生，從而促進結腸癌的生長和侵襲轉移，進一步影響結腸癌患者的預後。
목적：탐토Musashi 1재결장암중적표체여종류미혈관밀도（ MVD）급예후적관계。방법：응용실시형광정량PCR적방법검측36대결장암조직급기배대적정상결장조직중Musashi 1 mRNA적표체정황。통과면역조화법대96례결장암조직중Musashi 1표체정황화미혈관밀도（ MVD）진행검측。결합수방술후생존정황，통계분석Musashi 1표체여결장암병리삼수、예후급MVD지간적상관성。결과：실시형광정량PCR법결과현시，재결장암조직중，Musashi 1 mRNA적상대표체수평현저고우정상결장조직（P＜0．05）。면역조화결과현시，96례결장암조직중，유61례（63．5％）고표체Musashi 1。 Musashi 1적표체여환자성별、종류대소、분화정도무상관성（P＞0．05），이여종류적침윤심도、Dukes분기、림파전이、TNM분기밀절상관（ P＜0．05）。 Kaplan－Meier생존분석현시，Musashi 1고표체환자적5년생존솔현저저우Musashi 1저표체자（23．0％vs 60．0％；P＜0．01），차Musashi 1적표체여결장암혈관밀도상관（P＜0．01）。결론：결장암중Musashi 1적표체교정상암방조직고，구유통계학의의。결장암중Musashi 1적표체여종류침윤심도、Dukes분기、림파전이、TNM분기화혈관밀도밀절상관。 Musashi 1단백가통과촉진류내혈관증생，종이촉진결장암적생장화침습전이，진일보영향결장암환자적예후。
Objective:To investigate the Musashi 1 expression, and its correlation with clinical variables, such as clinical pathologic factors,angiogenesis and prognosis in colon cancer.Methods: Musashi 1 mRNA level was determined by quantitative real-time PCR( qRT-PCR) in 36 pairs of matched colon cancer tissues and adjacent non-tumorous tissues.The expression of Musashi 1 and mi-crovascular density ( MVD) were tested by immunohistochemical in 96 cases of colon cancer.Combined with postoperative follow-up survival situation, the correlation between the expression of Musashi 1, pathological parameters, prognosis and MVD were statistical analylised.Results:Musashi 1 mRNA level of colon cancer tissues determined by PCR was significantly higher than adjacent non-tumorous tissues( P<0.05).Positive Musashi 1 immunoreactivity was seen in 63.5%of colon cancer specimens,which was correlated with the depth of invasion,Duckes stage,lymph node metastasis,and TNM stage (P<0.05).Compared with Musashi 1 negative patients, Musashi 1 positive patients had significantly shorter survival time ( 23.0% vs 60.0%, P<0.01 ) .High intratumor blood microvessel density patients had significantly shorter survival times than low intratumor blood microvessel density patients(P<0.05).Intratumor blood microvessel density correlated with Musashi 1 expression (P<0.01).Conclusion:Musashi 1 mRNA level of colon cancer tissues was higher than adjacent non-tumorous tissues,the difference has statistically significant.We conclude that Musashi 1 expression correlates with the depth of invasion,Dukes stage,lymph node metastasis,and TNM stage and microvascular density ( MVD) in colon cancer.We propose that synthesized Musashi 1 increases intratumor angiogenesis,thus promotes tumor progression.Musashi 1 expression may be a good biomarker for poor prognosis in colon cancer.
