CAJ | 학술논문

目的：探讨青藤碱（Sinomenine，SIN）对脂多糖（LPS）以及白细胞介素4（IL－4）诱导的RAW264．7细胞向M1、M2型极化的影响。方法：以LPS刺激RAW264．7细胞诱导M1型极化，IL－4刺激RAW264．7细胞诱导M2型极化；青藤碱作用于LPS或IL－4诱导的巨噬细胞后：用酶联免疫法（ ELISA）检测不同诱导状态下RAW264．7细胞TNF－α和IL－10的分泌量；荧光定量PCR检测与巨噬细胞极化相关的精氨酸酶－1（Arg－1）、一氧化氮合酶（iNOS）、细胞因子信号转导抑制蛋白－2（SOCS2）和细胞因子信号转导抑制蛋白－3（SOCS3）的mRNA表达水平。结果：青藤碱能抑制LPS诱导下细胞TNF－α的分泌量，抑制细胞iNOS和SOCS3的mRNA表达水平的升高。青藤碱能抑制IL－4诱导下细胞IL－10的分泌量和Arg1的mRNA表达水平的升高，对IL－4诱导下细胞SOCS2的mRNA表达水平的升高没有明显影响。结论：青藤碱对LPS诱导下巨噬细胞向M1型极化具有抑制作用；对IL－4诱导下巨噬细胞向M2型极化具有抑制作用。青藤碱对M1／M2亚型的失衡具有调节作用，有利于维持其动态平衡。
목적：탐토청등감（Sinomenine，SIN）대지다당（LPS）이급백세포개소4（IL－4）유도적RAW264．7세포향M1、M2형겁화적영향。방법：이LPS자격RAW264．7세포유도M1형겁화，IL－4자격RAW264．7세포유도M2형겁화；청등감작용우LPS혹IL－4유도적거서세포후：용매련면역법（ ELISA）검측불동유도상태하RAW264．7세포TNF－α화IL－10적분비량；형광정량PCR검측여거서세포겁화상관적정안산매－1（Arg－1）、일양화담합매（iNOS）、세포인자신호전도억제단백－2（SOCS2）화세포인자신호전도억제단백－3（SOCS3）적mRNA표체수평。결과：청등감능억제LPS유도하세포TNF－α적분비량，억제세포iNOS화SOCS3적mRNA표체수평적승고。청등감능억제IL－4유도하세포IL－10적분비량화Arg1적mRNA표체수평적승고，대IL－4유도하세포SOCS2적mRNA표체수평적승고몰유명현영향。결론：청등감대LPS유도하거서세포향M1형겁화구유억제작용；대IL－4유도하거서세포향M2형겁화구유억제작용。청등감대M1／M2아형적실형구유조절작용，유리우유지기동태평형。
Objective:To investigate sinomenine (Sinomenine,SIN) effect on RAW264.7 cells polarization to M1 or M2 phenotype induced by lipopolysaccharide (LPS) or interleukin-4 (IL-4) .Methods:RAW264.7 cells were induced to polarize to M1 by LPS ,and to M2 by IL-4.Sinomenine effects on LPS or IL-4 induced macrophages:TNF-αand IL-10 secretion induced by different condition were detected by Enzyme linked immunosorbent assay (ELISA);The expression level of mRNA of Arginase1(Arg-1),Nitric oxide synthase(iNOS),suppressor of cytokine signaling protein-2(SOCS2) and suppressor of cytokine signaling protein-3(SOCS3) of M1/M2 phenotypes were detected by real time PCR respectively.Results:Sinomenine inhibited the increase of TNF-αsecretion,iNOS and SOCS3 mRNA expression level induced by LPS.Sinomenine inhibited the increase of IL-10 secretion and Arg-1 mRNA expression level induced by IL-4,but SOCS2 mRNA expression level was not affected by Sinomenine.Conclusion: Sinomenine can inhibite the macrophage polarization to M1 and M2 induced by LPS and IL-4.Sinomenine plays a regulatory role on imbalance of M1/M2,and is conducive to maintain the dynamic balance.