CAJ | 학술논문

目的：检测肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）在颅内动脉瘤中的表达情况。方法回顾性连续纳入经DSA证实为颅内动脉瘤，并经显微神经外科手术夹闭动脉瘤的患者共16例（动脉瘤组）。对照组为同期经CT及MRI证实为非血管性疾病的外伤患者，共19例。经苏木素-伊红（HE）染色及免疫组化染色，检测动脉瘤的瘤壁组织和正常血管壁中TNF-α、IL-6的着色部位，分析其表达染色后的平均光密度值，并进行染色强度的比较。结果（1）对照组的动脉壁各层均无明显TNF-α、IL-6的阳性表达。动脉瘤组瘤壁组织的内膜、中膜、外膜均有TNF-α、IL-6的阳性表达。（2）动脉瘤组患者TNF-α、IL-6的平均光密度值分别为0.182±0.069和0.148±0.062，高于对照组的0.144±0.031和0.105±0.020，差异有统计学意义（均P＜0.05）。（3）动脉瘤壁内、中、外膜各层TNF-α表达的平均光密度值分别为0.224±0.071、0.134±0.040、0.106±0.065，差异有统计学意义（P＜0.01）。（4）动脉瘤壁外膜IL-6表达的平均光密度值低于中膜、内膜（0.096±0.018比0.145±0.050、0.148±0.070），差异有统计学意义（P＜0.05）。（5）Spearman相关分析结果显示，动脉瘤组TNF-α的平均光密度值与IL-6的平均光密度值呈正相关（r＝0.452，P＜0.05）。结论 TNF-α、IL-6在动脉瘤的瘤壁组织中表达较高，可能参与了颅内动脉瘤的生成和破裂。
목적：검측종류배사인자α（TNF-α）、백세포개소6（IL-6）재로내동맥류중적표체정황。방법회고성련속납입경DSA증실위로내동맥류，병경현미신경외과수술협폐동맥류적환자공16례（동맥류조）。대조조위동기경CT급MRI증실위비혈관성질병적외상환자，공19례。경소목소-이홍（HE）염색급면역조화염색，검측동맥류적류벽조직화정상혈관벽중TNF-α、IL-6적착색부위，분석기표체염색후적평균광밀도치，병진행염색강도적비교。결과（1）대조조적동맥벽각층균무명현TNF-α、IL-6적양성표체。동맥류조류벽조직적내막、중막、외막균유TNF-α、IL-6적양성표체。（2）동맥류조환자TNF-α、IL-6적평균광밀도치분별위0.182±0.069화0.148±0.062，고우대조조적0.144±0.031화0.105±0.020，차이유통계학의의（균P＜0.05）。（3）동맥류벽내、중、외막각층TNF-α표체적평균광밀도치분별위0.224±0.071、0.134±0.040、0.106±0.065，차이유통계학의의（P＜0.01）。（4）동맥류벽외막IL-6표체적평균광밀도치저우중막、내막（0.096±0.018비0.145±0.050、0.148±0.070），차이유통계학의의（P＜0.05）。（5）Spearman상관분석결과현시，동맥류조TNF-α적평균광밀도치여IL-6적평균광밀도치정정상관（r＝0.452，P＜0.05）。결론 TNF-α、IL-6재동맥류적류벽조직중표체교고，가능삼여료로내동맥류적생성화파렬。
Objective Todetecttheexpressionlevelsoftumornecrosisfactor-α(TNF-α)and interleukin-6(IL-6)inintracranialaneurysms.Methods Sixteenconsecutivepatients(aneurysm group)with intracranial aneurysm confirmed by digital subtraction angiography (DSA)and clipped by microneurosurgery were enrolled retrospectively. A total of 19 trauma patients without vascular disease confirmed by CT and magnetic resonance imaging (MRI)in the same period were used as a control group. Hematoxylin-eosin (HE)staining and immunohistochemical staining were used to detect the aneurysm wall tissue and the colored portions of TNF-α and IL-6 in normal vessel wall,the mean value of optical density after its expression was analyzed,and the intensity of staining was compared. Results (1)Each layer of artery walls of the control group had no obvious TNF-α and IL-6 expression. The inner,media and out membranes of the aneurysm wall tissue of the aneurysm group had positive expression of TNF-αand IL-6. (2)The mean optical densities of TNF-α and IL-6 in patients of the aneurysm group were 0. 182 ± 0. 069 and 0. 148 ± 0.062 respectively,and they were higher than 0. 144 ± 0. 031 and 0. 105 ± 0. 020 of the control group. The differences were statistically significant (all P<0. 05). (3)The mean optical densities of TNF-α expression of each layer of the inner,media and out membranes in the aneurysm walls were 0. 224 ± 0. 071,0. 134 ± 0. 040,and 0. 106 ± 0. 065,respectively. There were significant differences (P<0.01). (4)The mean optical density expressed by IL-6 in the out membrane of the aneurysm walls was lower than the media and inner membranes (0. 096 ± 0. 018 vs. 0. 145 ± 0. 050,and 0. 148 ± 0. 070). There were significant differences (P<0. 05). (5)The results of Spearman correlation analysis showed that the mean optical density of TNF-αof the aneurysm group was positively correlated with that of IL-6 (r=0. 452, P<0.05).Conclusion TheexpressionlevelsofTNF-αandIL-6intheaneurysmwalltissueare higher,and they may be involved in intracranial aneurysm formation and rupture.