CAJ | 학술논문

目的：探究铜绿假单胞菌在生物膜状态与浮游状态时对常见抗菌药物的耐药性差异，为临床合理用药提供依据。方法在体外培养基中对铜绿假单胞菌进行孵育处理，并分为浮游液对照培养组（A组）、放置生物膜培养后的培养液组（B组）和生物膜培养硅胶片粘附组（C组）。取浮游培养（培养液）、生物膜培养（培养液）以及生物膜培养（硅胶片粘附）铜绿假单胞菌进行银染色、胞外糖染色并且扫描电镜鉴定。向3组培养基中加入不同抗菌药物，利用K-B方法观察细菌耐药性情况。结果 B组与A组药物耐药率和最低抑菌浓度（MIC）值差异均无统计学意义（p>0.05）；C组菌株与A组比较，对美洛培南（50.00%vs 26.47%）、头孢吡肟（53.52%vs 25.00%）、头孢他啶（60.94% vs 20.59%）、头孢曲松（70.31% vs 61.76%）、左氧氟沙星（39.84%vs 2.94%）及哌拉西林（48.05%vs 8.82%）的耐药性差异均有统计学意义（ p<0.01）。C组最低生物膜清除浓度（ MBEC）值是A组和B组MIC值的100倍左右。结论体外建立细菌生物膜简便可行，通过银染法联合电镜可观察生物膜形成情况；生物膜会改变细菌的理化特性，提高对抗菌药物的耐药性，增加临床治疗难度。
목적：탐구동록가단포균재생물막상태여부유상태시대상견항균약물적내약성차이，위림상합리용약제공의거。방법재체외배양기중대동록가단포균진행부육처리，병분위부유액대조배양조（A조）、방치생물막배양후적배양액조（B조）화생물막배양규효편점부조（C조）。취부유배양（배양액）、생물막배양（배양액）이급생물막배양（규효편점부）동록가단포균진행은염색、포외당염색병차소묘전경감정。향3조배양기중가입불동항균약물，이용K-B방법관찰세균내약성정황。결과 B조여A조약물내약솔화최저억균농도（MIC）치차이균무통계학의의（p>0.05）；C조균주여A조비교，대미락배남（50.00%vs 26.47%）、두포필우（53.52%vs 25.00%）、두포타정（60.94% vs 20.59%）、두포곡송（70.31% vs 61.76%）、좌양불사성（39.84%vs 2.94%）급고랍서림（48.05%vs 8.82%）적내약성차이균유통계학의의（ p<0.01）。C조최저생물막청제농도（ MBEC）치시A조화B조MIC치적100배좌우。결론체외건립세균생물막간편가행，통과은염법연합전경가관찰생물막형성정황；생물막회개변세균적이화특성，제고대항균약물적내약성，증가림상치료난도。
Objective To compare antibiotic resistance of pseudomonas aeruginosa in biofilms and in planktonic state,so as to provide practical guidance and theoretical support for clinical treatment of drug. Methods pseudomonas aeruginosa were divided into control culture group( group A),medium group of biological membrane after culture( group B) and the biofilm culturing silica film adhesion group( group C). Three groups were observed by silver staining,extracellular sugar staining and scanning of electron microscope. These groups were cultured with different antibiotic medium,and the situation of bacterial resistance was observed by the method of k-B. Results There was no significant difference in drug resistance and minimum inhibitory concentration(MIC)between group A and B(p >0. 01). Compared with group A, group C showed that their drug resistance on meropenem,cefepime,ceftazidime,ceftriaxone,amoxicillin and levofloxacin were much higher(p<0. 01). The minimal biofilm eradication concentration(MBEC)of group C was about 100 times than that of group A and group B. Conclusion The assay of establishment of pseudomonas aeruginosa biofilm and the methods observed by silver stainingin vitro is convenient and feasible. At the same time,pseudomonas aeruginosa in biofilm exhibits far more resistance to antimicrobial than its planctonic counterparts does.