CAJ | 학술논문

目的：探讨重组人促红细胞生成素（ rHuEPO ）治疗大鼠肾脏缺血再灌注性损伤的最佳剂量及时机。方法将60只SD大鼠随机分为6组，每组10只，假手术组、模型组、高剂量24（高24）组：造模后腹腔注射rHuEPO 5000 U／kg，24 h后取肾标本；低剂量24（低24）组：rHuEPO 2500 U／kg，24 h取肾脏标本；高剂量48（高48）组：rHuEPO 5000 U／kg，48 h取肾标本；低剂量48（低48）组：rHuEPO 2500 U／kg，24 h后追加2500 U／kg，48 h取肾标本。检测血清尿素氮（BUN）、肌酐水平（Scr）、肾组织中磷酸化应激活化蛋白激酶（p-JNK）的表达及肾小管上皮细胞凋亡情况。结果与假手术组相比，其他5组大鼠BUN、Scr明显升高，凋亡加重，p-JNK表达增多；与模型组相比，各rHuEPO治疗组肾功能明显好转，肾功能高24组好于低24组，高48组好于低48组（P＜0．05）。结论及时一次大剂量应用rHuEPO对大鼠肾脏具有更好的保护作用，可能是通过调节p-JNK等因子表达以减少凋亡的发生，而达到改善肾脏病理性损伤。
목적：탐토중조인촉홍세포생성소（ rHuEPO ）치료대서신장결혈재관주성손상적최가제량급시궤。방법장60지SD대서수궤분위6조，매조10지，가수술조、모형조、고제량24（고24）조：조모후복강주사rHuEPO 5000 U／kg，24 h후취신표본；저제량24（저24）조：rHuEPO 2500 U／kg，24 h취신장표본；고제량48（고48）조：rHuEPO 5000 U／kg，48 h취신표본；저제량48（저48）조：rHuEPO 2500 U／kg，24 h후추가2500 U／kg，48 h취신표본。검측혈청뇨소담（BUN）、기항수평（Scr）、신조직중린산화응격활화단백격매（p-JNK）적표체급신소관상피세포조망정황。결과여가수술조상비，기타5조대서BUN、Scr명현승고，조망가중，p-JNK표체증다；여모형조상비，각rHuEPO치료조신공능명현호전，신공능고24조호우저24조，고48조호우저48조（P＜0．05）。결론급시일차대제량응용rHuEPO대대서신장구유경호적보호작용，가능시통과조절p-JNK등인자표체이감소조망적발생，이체도개선신장병이성손상。
Objective To explore the optimal dosing and timing of recombinant human erythropoietin ( rHuEPO) for the treatment of renal ischemia-reperfusion injury in rats .Methods Sixty SD rats were randomly divided into six groups ,with 10 rats in each group ,including sham operation group ,ischemia reperfusion injury group ( model group ) , high-dose 24 group,low-dose 24 group,high-dose 48 group,and low-dose 48 group.The rats in the high-dose 24 group received the injection of rHuEPO at a dose of 5000 U/kg and then renal specimen sampling 24 hours later.The rats in the low-dose 24 group received the injection of rHuEPO at a dose of 2 500 U/kg and then renal specimen sampling 24 hours later .The rats in the high-dose 48 group received the injection of rHuEPO at a dose of 5 000 U/kg and then renal specimen sampling 48 hours later .The rats in the low-dose 48 group received the injection of rHuEPO at a dose of 2 500 U/kg,and then were give an additional injection of rHuEPO at a dose of 2500 U/kg 24 hours later,followed by the renal specimen sampling 48 hours later.To detect the levels of blood urea nitrogen (BUN) and serum creatinine (Scr),the expression of phosphorylated c-Jun N-terminal kinase(p-JNK) in the kidney tissues and the renal tubular cell apoptosis .Results Compared with sham group ,the rats in other 5 groups showed the significantly higher levels of BUN and Scr ,apoptosis increased ,and p-JNK expression increased .Compared with model group ,the rats in the other groups with rHuEPO therapy showed a significant improvment of renal function .The renal function of rats in the high-dose 24 group was superior to that in the low-dose 24 group,and the renal function of rats in the high-dose 48 group was superior to that in the low-dose 48 group(P<0.05).Conclusion The timely and sufficient large-dose of rHuEPO has a protective effect on rats′kidney, which might contribute to the improvement of renal pathological injury by regulating the p-JNK expression and decreasing the apoptosis .