CAJ | 학술논문

目的：探讨涤毒灌肠方对5/6肾切除大鼠血、尿硫酸吲哚酚含量的影响。方法 SD大鼠80只，随机抽取8只作为空白对照组，其余72只行5/6肾切除术复制慢性肾衰模型，将造模成功的60只大鼠随机分为模型对照组、药用炭治疗组、涤毒灌肠方高、中、低剂量组，用药周期为56 d。测量大鼠血清肌酐、尿素氮，高效液相色谱-质谱分析法测定血、尿硫酸吲哚酚的含量。结果灌肠方各剂量组和药用炭组血硫酸吲哚酚含量明显下降(P<0.05)，且高、中剂量组比药用炭组下降更为明显(P<0.05)；灌肠方高、中剂量组、药用炭组尿硫酸吲哚酚含量明显下降( P<0.05)，且高剂量组比药用炭组更为明显( P<0．05)。结论涤毒灌肠方降低5/6肾切除大鼠血、尿中硫酸吲哚酚含量可能是其干预肾衰竭的作用机制之一。
목적：탐토조독관장방대5/6신절제대서혈、뇨류산신타분함량적영향。방법 SD대서80지，수궤추취8지작위공백대조조，기여72지행5/6신절제술복제만성신쇠모형，장조모성공적60지대서수궤분위모형대조조、약용탄치료조、조독관장방고、중、저제량조，용약주기위56 d。측량대서혈청기항、뇨소담，고효액상색보-질보분석법측정혈、뇨류산신타분적함량。결과관장방각제량조화약용탄조혈류산신타분함량명현하강(P<0.05)，차고、중제량조비약용탄조하강경위명현(P<0.05)；관장방고、중제량조、약용탄조뇨류산신타분함량명현하강( P<0.05)，차고제량조비약용탄조경위명현( P<0．05)。결론조독관장방강저5/6신절제대서혈、뇨중류산신타분함량가능시기간예신쇠갈적작용궤제지일。
Objective To study the effect of Didu decoction enema therapy on indoxyl sulfate content in serum and urine in 5/6 nephrectomy rats. Methods Eight SD rats were randomly selected as the control group and 72 SD rats were carried on 5/6 nephrectomy. Sixty rats,which were successfully modeled,were divided into the pathological control group,the medicinal charcoal tablets treatment group and three Didu enema treatment groups with high,middle and low doses. After 56 days treatment,the creatinine and urea nitrogen content in serum were measured, and the content of indoxyl sulfate in serum and urine was determined by HPLC-MS. Results The contents of creatinine and urea nitrogen in serum and the level in serum indoxyl sulfate were significantly decreased in the Didu decoction enema therapy groups and the medicinal charcoal tablets treatment group (P<0.05). Moreover,the high and middle dose groups were better than the medicinal charcoal tablets treatment group ( P<0.05) . The content of urinary indoxyl sulfate decreased significantly in high and middle dose groups and the medicinal charcoal tablets treatment group (P<0.05). Moreover,the high dose group was better than the medicinal charcoal tablets treatment group ( P<0.05) . Conclusion Didu decoction enema may play a protective effect on renal failure by decreasing the content of indoxyl sulfate content in serum and urine.