CAJ | 학술논문

目的：探讨定经汤主要药对菟丝子-柴胡调节卵巢早衰的作用机制。方法 SD大鼠随机分为4组：正常大鼠组、模型组、定经汤组和菟丝子-柴胡组；分别检测4组大鼠血清中与卵巢早衰相关的生物分子：β-EP、IL-1、NOS、NO、ER、PR、GnRH、CRH、FSH、LH、E2、P、ACTH和CORT；利用因子分析方法探究菟丝子-柴胡干预下各生物分子之间的相互调控关系。结果菟丝子-柴胡干预状态生殖内分泌网络主调控因子主要包括垂体-卵巢轴4个生物分子、垂体-肾上腺轴靶腺层生物分子CORT以及部分下丘脑生物分子；GnRH是菟丝子-柴胡干预状态主调控因子与其他公共因子共有的生物分子。结论菟丝子-柴胡一方面可以直接调节垂体-肾上腺轴生物分子，另一方面又可以通过下丘脑间接调节垂体-肾上腺轴生物分子；GnRH是各调控因子之间的枢纽生物分子。
목적：탐토정경탕주요약대토사자-시호조절란소조쇠적작용궤제。방법 SD대서수궤분위4조：정상대서조、모형조、정경탕조화토사자-시호조；분별검측4조대서혈청중여란소조쇠상관적생물분자：β-EP、IL-1、NOS、NO、ER、PR、GnRH、CRH、FSH、LH、E2、P、ACTH화CORT；이용인자분석방법탐구토사자-시호간예하각생물분자지간적상호조공관계。결과토사자-시호간예상태생식내분비망락주조공인자주요포괄수체-란소축4개생물분자、수체-신상선축파선층생물분자CORT이급부분하구뇌생물분자；GnRH시토사자-시호간예상태주조공인자여기타공공인자공유적생물분자。결론토사자-시호일방면가이직접조절수체-신상선축생물분자，령일방면우가이통과하구뇌간접조절수체-신상선축생물분자；GnRH시각조공인자지간적추뉴생물분자。
Objective To explore the biological mechanisms of Dingjing decoction herbs couples of Cuscuta Semen-Bupleuri Radix on the premature ovarian failure ( POF). Methods 120 SD rats were randomly divided into four groups including the normal group,the model group,the Dingjing Decoction group and the Cuscuta Semen-Bupleuri Radix group. The biomolecules associated with POF such as β-EP,IL-1,NOS, NO,ER, PR, GnRH, CRH, FSH, LH, E2, P, ACTH and CORT were detected. Regulation relations of biological molecules were explored based on factor analysis.Results The master regulatory factors regulated by Cuscuta Semen-Bupleuri Radix mainly contained the pituitary-ovarian axis four biomolecules, the pituitary-adrenal axis biomolecule CORT and part of hypothalamus biomolecules. GnRH was the common biomolecule in the hypothalamus part of the first and the other common factors regulated by Cuscuta Semen-Bupleuri Radix. Conclusion Cuscuta Semen-Bupleuri Radix can directly modulate pituitary-adrenal axis molecules,as well as indirectly modulate the pituitary-adrenal axis molecules through hypothalamus. GnRH plays a pivotal role in these factors.