CAJ | 학술논문

目的：应用1 H-NMR法研究RAW264.7细胞不同炎症状态与细胞膜磷脂成分变化的相关性。方法RAW264.7细胞随机分为空白组、炎症组、双氯芬酸钠组、尼美舒利组、美洛昔康组,每组5皿细胞。采用100 ng·mL-1 KLA诱发RAW264.7细胞产生炎症,50μg·mL-1双氯芬酸钠、45μg·mL-1尼美舒利、30μg·mL-1美洛昔康分别干预建立给药组模型,TNF-α生成量变化验证模型,1 H-NMR法采集各组细胞膜磷脂的氢谱,主成分分析法( PCA)分析细胞膜磷脂变化。结果细胞模型建立成功,1 H-NMR图谱与PCA结合分析比较细胞膜磷脂,空白组、双氯芬酸钠组、尼美舒利组、美洛昔康组分别与炎症组距离较远,得分图分型较好,各组细胞膜磷脂差异均有统计学意义。结论 RAW264.7细胞膜磷脂在炎症前后发生了显著的变化,炎症的发生与磷脂的变化具有相关性。
목적：응용1 H-NMR법연구RAW264.7세포불동염증상태여세포막린지성분변화적상관성。방법RAW264.7세포수궤분위공백조、염증조、쌍록분산납조、니미서리조、미락석강조,매조5명세포。채용100 ng·mL-1 KLA유발RAW264.7세포산생염증,50μg·mL-1쌍록분산납、45μg·mL-1니미서리、30μg·mL-1미락석강분별간예건립급약조모형,TNF-α생성량변화험증모형,1 H-NMR법채집각조세포막린지적경보,주성분분석법( PCA)분석세포막린지변화。결과세포모형건립성공,1 H-NMR도보여PCA결합분석비교세포막린지,공백조、쌍록분산납조、니미서리조、미락석강조분별여염증조거리교원,득분도분형교호,각조세포막린지차이균유통계학의의。결론 RAW264.7세포막린지재염증전후발생료현저적변화,염증적발생여린지적변화구유상관성。
Objective To explore the relevance between inflammation and phospholipids in RAW264.7 cells by1 H-NMR. Methods RAW264. 7 cells were divided into five groups, including the blank group, the inflammation group, the diclofenac sodium group, the nimesulide group and the meloxicam group. The inflammatory stimulation was established by 100 ng · mL-1 of KLA treatment. 50 μg · mL-1 diclofenac sodium,45 μg · mL-1 nimesulide and 30 μg · mL-1 meloxicam were added into the different groups, respectively. The levels of TNF-αin culture supernatant were measured. The changes of phospholipids were detected by 1H-NMR spectra coupled with principal component analysis ( PCA). Results A separation tendency of phospholipids was observed in the blank group and three treatment groups when compared with the inflammation group.Conclusion The phospholipids in RAW264.7 cells may be changed before and after inflammatory stimulation.