CAJ | 학술논문

目的：建立金钗石斛HPLC的特征图谱，为其质量评价提供参考。方法采用ZORBAX SB-Aq色谱柱；流动相乙腈-0．2%(体积分数)甲酸，梯度洗脱，流速1．0 mL · min-1；柱温30℃；检测波长270 nm。结果分别建立了不同产地金钗石斛的HPLC特征图谱，一类是以贵州赤水产金钗石斛为代表的HPLC特征图谱，标记出22个共有峰，相似度为0．979~0．998；一类是以云南产金钗石斛为代表的HPLC特征图谱，标记出27个共有峰，相似度为0．902~0．980。结论金钗石斛的HPLC特征图谱主要分为2类，贵州赤水产为一类，云南产为一类，广西产的两类都有。本法稳定可靠、重复性好，可用于不同产地金钗石斛的鉴别和质量控制。
목적：건립금차석곡HPLC적특정도보，위기질량평개제공삼고。방법채용ZORBAX SB-Aq색보주；류동상을정-0．2%(체적분수)갑산，제도세탈，류속1．0 mL · min-1；주온30℃；검측파장270 nm。결과분별건립료불동산지금차석곡적HPLC특정도보，일류시이귀주적수산금차석곡위대표적HPLC특정도보，표기출22개공유봉，상사도위0．979~0．998；일류시이운남산금차석곡위대표적HPLC특정도보，표기출27개공유봉，상사도위0．902~0．980。결론금차석곡적HPLC특정도보주요분위2류，귀주적수산위일류，운남산위일류，엄서산적량류도유。본법은정가고、중복성호，가용우불동산지금차석곡적감별화질량공제。
Objective To establish HPLC characteristic spectrum and provide reference for the quality evaluation of Dendrobium nobile Lindl..Methods Chromatographic separation was performed on ZORBAX SB-Aq column at 30℃ eluted with acetonitrile and 0. 2%methanoic acid in a gradient elution at a flow rate of 1. 0 mL·min-1 . The detection wavelength was set at 270 nm. Results The HPLC characteristic spectrum of D. nobile from different regions were constructed. One represented the characteristic spectrum of D.nobile from Chishui,Guizhou province with 22 common specific peaks,and the similarities were 0. 979-0. 998. The other represented the characteristic spectrum of D. nobile from Yunnan province with 27 common specific chromatographic peaks,and the similarities were 0. 902-0. 980. Conclusion HPLC characteristic spectrum of D.nobile could be divided into two major classes,one was from Chishui,Guizhou Province,and the other was from Yunnan Province. Samples from Guangxi include this two major classes. The method was reliable with good reproducibility. It could be used for the identification and quality control of D.nobile from different regions.