CAJ | 학술논문

目的：采用叶绿体基因psbA-trnH间隔区序列探讨广藿香及其常见伪品的分子鉴定方法。方法分别提取广藿香及藿香、广防风植物的总DNA,经PCR扩增psbA-trnH间隔区序列,产物纯化后测序,采用Clustal_X 1.8和MEGA 5.05软件对序列进行分析。结果广藿香与藿香、广防风的最小种间K2P 距离(0.1592)大于最大种内K2P距离(0.0043),邻接树中广藿香9个不同产地来源样品聚为一支。广藿香与藿香、广防风之间存在多个特异性信息位点。结论 psbA-trnH序列可作为广藿香及藿香、广防风分子鉴定的依据。
목적：채용협록체기인psbA-trnH간격구서렬탐토엄곽향급기상견위품적분자감정방법。방법분별제취엄곽향급곽향、엄방풍식물적총DNA,경PCR확증psbA-trnH간격구서렬,산물순화후측서,채용Clustal_X 1.8화MEGA 5.05연건대서렬진행분석。결과엄곽향여곽향、엄방풍적최소충간K2P 거리(0.1592)대우최대충내K2P거리(0.0043),린접수중엄곽향9개불동산지래원양품취위일지。엄곽향여곽향、엄방풍지간존재다개특이성신식위점。결론 psbA-trnH서렬가작위엄곽향급곽향、엄방풍분자감정적의거。
Objective To perform the molecular identification of Pogostemon cablin, Agastache rugosus and Epimeredi indica based on psbA-trnH intergenic spacer sequences. Methods Total DNA was extracted and psbA-trnH intergenic spacer sequences were amplified by PCR.PCR products were directly sequenced after purification.Sequences were analyzed with Clustal_X 1.8 and MEGA 5.05 software. Results The minimum inter-specific K2P distance was 0.159 2, higher than the maximum intra-specific K2P distance (0.004 3). The different samples from nine origin places of P. cablin were clustered into one clade in the Neighbor-Joining tree. Distinctive variation sites between P. cablin, A. rugosus and E. indica could be identified. Conclusion The psbA-trnH intergenic spacer sequences can be used to identificate P. cablin, A. rugosus and E. indica in molecule level.