CAJ | 학술논문

目的：观察五味子乙素B（ Schisandrin B ， Sch B）对人胃癌细胞MGC－803增殖、迁移能力及基质金属蛋白酶－9（matrix metalloproteinase 9， MMP－9）mRNA表达的影响，并探讨其可能的分子机制。方法：采用CCK－8法检测50、100、200、400和800μmol／L Sch B对胃癌细胞增殖的抑制作用；光镜观察细胞生长状态变化，并计数细胞分裂指数；细胞划痕实验检测200μmol／L Sch B对细胞迁移能力的影响；定量PCR检测细胞MMP－9 mRNA的表达。结果：CCK－8结果显示，Sch B对胃癌细胞的增殖具有显著抑制作用，且呈剂量和时间依赖性；胃癌细胞经不同浓度Sch B处理24、48、72 h，光镜下可见大量细胞皱缩，胞膜界限模糊，核质浓缩及凋亡小体形成，细胞分裂指数随Sch B剂量增加和作用时间延长而降低；200μmol／L Sch B作用24、48、72 h，细胞迁移率分别为（39．57±9．14）％，（56．57±10．04）％和（68．21±11．43）％，明显低于对照组（P＜0．05）；PCR结果显示，胃癌细胞中MMP－9 mRNA表达显著低于对照组。结论：Sch B对胃癌细胞的增殖抑制呈剂量和浓度依赖性，并降低胃癌细胞迁移能力，可能与下调MMP－9基因的表达有关。
목적：관찰오미자을소B（ Schisandrin B ， Sch B）대인위암세포MGC－803증식、천이능력급기질금속단백매－9（matrix metalloproteinase 9， MMP－9）mRNA표체적영향，병탐토기가능적분자궤제。방법：채용CCK－8법검측50、100、200、400화800μmol／L Sch B대위암세포증식적억제작용；광경관찰세포생장상태변화，병계수세포분렬지수；세포화흔실험검측200μmol／L Sch B대세포천이능력적영향；정량PCR검측세포MMP－9 mRNA적표체。결과：CCK－8결과현시，Sch B대위암세포적증식구유현저억제작용，차정제량화시간의뢰성；위암세포경불동농도Sch B처리24、48、72 h，광경하가견대량세포추축，포막계한모호，핵질농축급조망소체형성，세포분렬지수수Sch B제량증가화작용시간연장이강저；200μmol／L Sch B작용24、48、72 h，세포천이솔분별위（39．57±9．14）％，（56．57±10．04）％화（68．21±11．43）％，명현저우대조조（P＜0．05）；PCR결과현시，위암세포중MMP－9 mRNA표체현저저우대조조。결론：Sch B대위암세포적증식억제정제량화농도의뢰성，병강저위암세포천이능력，가능여하조MMP－9기인적표체유관。
Objective:To investigate the effects of Schisandrin B (Sch B)on the proliferation, migration and MMP-9 expression in human gastric cancer cells and to discuss the possible mechanism .Methods:CCK-8 assay was used to detect cell relative viability af-ter treatment with Sch B for different concentration (50、100、200、400和800μmol/L).The morphologic changes of gastric cancer cells were observed and the mitosis index was assessed by inverted light microscope .Cell migration was determined by scratch assay when the cells treated with 200μmol/L Sch B.Quantitative PCR was applied to examination of the mRNA of MMP -9 in human gastric cancer cells .Results:CCK-8 assay showed that Sch B , in a dose-and time dependent manner , inhibited the growth of MGC -803 cells.After 24、48、72 h treated with Sch B , a large number of shrinkage cells can be seen under light microscope , accompanied with nuclear envelope disappears , nuclear chromatin condensation and apoptotic body formation , and the mitosis index decreased with a dose-and time dependent manner .The scratch assay showed that, the migration rates were(39.57 ±9.14)%, (56.57 ±10.04)% and (68.21 ±11.43) %respectively after 24、48、72 h treated with 200μmol/L Sch B,which were significantly slower than those in the control group (P<0.05).PCR results showed that MMP-9 mRNA expression was significantly decreased in gastric cancer cells after treated with 200μmol/L Sch B for 24, 48 and 72 h.Conclusion:Sch B inhibits gastric cancer cells proliferation in a dose -and time dependent manner , and dreases the migration ability , which may be attributed to the down -regulation of the MMP-9 mRNA expres-sion.