CAJ | 학술논문

目的：探讨自体骨髓基质干细胞（BMSCs）移植联合表皮生长因子受体（EGFR）抑制剂对大鼠脊髓损伤（SCI）治疗的效果。方法：培养与纯化骨髓基质干细胞。60只SD大鼠制备脊髓损伤模型，随机分为4组：基质干细胞组、EGFR抑制剂组、联合组和对照组。采用BBB法进行运动功能评分和切片观察来检测修复情况。结果：随着时间的延长，四个组的大鼠的运动功能都是逐渐恢复，术后第7天起，对照组BBB评分明显低于其余三个组（P＜0．05），且联合组大鼠的神经功能恢复好于基质干细胞组和表皮生长因子受体抑制剂组（P＜0．05）。组织学染色显示三个实验组脊髓组织恢复均好于对照组（P＜0．05）；联合治疗组脊髓组织恢复好于基质干细胞组与表皮生长因子受体抑制剂组（P＜0．05）。结论：骨髓基质干细胞联合EG-FR抑制剂具有促进大鼠脊髓损伤后神经功能修复的作用。
목적：탐토자체골수기질간세포（BMSCs）이식연합표피생장인자수체（EGFR）억제제대대서척수손상（SCI）치료적효과。방법：배양여순화골수기질간세포。60지SD대서제비척수손상모형，수궤분위4조：기질간세포조、EGFR억제제조、연합조화대조조。채용BBB법진행운동공능평분화절편관찰래검측수복정황。결과：수착시간적연장，사개조적대서적운동공능도시축점회복，술후제7천기，대조조BBB평분명현저우기여삼개조（P＜0．05），차연합조대서적신경공능회복호우기질간세포조화표피생장인자수체억제제조（P＜0．05）。조직학염색현시삼개실험조척수조직회복균호우대조조（P＜0．05）；연합치료조척수조직회복호우기질간세포조여표피생장인자수체억제제조（P＜0．05）。결론：골수기질간세포연합EG-FR억제제구유촉진대서척수손상후신경공능수복적작용。
Objective:To evaluate the effects of transplantation of bone marrow stromal cells (BMSCs) with inhibitors of the epider-mal growth factor receptor ( EGFR) therapy after rats spinal cordinjury ( SCI).Methods:BMSCs were cultured and purified in vitro . The rat model was prepared with modified Alle method .Sixty rats were devided into four groups:stem cell group , inhibitors of EGFR group, combination group and control group.The motor function was evaluated with BBB score and the neurological function recovery were observed .Results:Statistical differences were showed in inter group.Compared with control group , in practical group ,the motor function and morphological recovery was obvious in MSCs combined EGFR inhibitors group .Conclusion:The neurological function of SCI rats can be obviously improved by BMSCs transplantation combined with Inhibitors of the epidermal growth factor receptor .