CAJ | 학술논문

目的：研究金雀异黄素能否和如何抑制肝细胞癌MHCC97H细胞系肝癌干细胞样细胞侵袭。方法：干细胞条件培养基悬浮培养MHCC97 H细胞系球形成细胞，作为肝癌干细胞样细胞。不同浓度金雀异黄素处理后，Transwell小室侵袭试验检测体外细胞侵袭能力；Western blot分析Gli1和Snail1蛋白表达。结果：干细胞条件培养基悬浮培养MHCC97H细胞能形成肿瘤球。金雀异黄素（5、10和20μM）作用第3代球形成细胞即肝癌干细胞样细胞72 h降低肿瘤球形成率和细胞侵袭率；呈浓度依赖性。金雀异黄素（5、10和20μM）孵育肝癌干细胞样细胞24 h下调Gli1和Snail1蛋白表达。结论：金雀异黄素可能通过调控Gli1抑制Snail1蛋白表达抑制肝癌干细胞样细胞侵袭。
목적：연구금작이황소능부화여하억제간세포암MHCC97H세포계간암간세포양세포침습。방법：간세포조건배양기현부배양MHCC97 H세포계구형성세포，작위간암간세포양세포。불동농도금작이황소처리후，Transwell소실침습시험검측체외세포침습능력；Western blot분석Gli1화Snail1단백표체。결과：간세포조건배양기현부배양MHCC97H세포능형성종류구。금작이황소（5、10화20μM）작용제3대구형성세포즉간암간세포양세포72 h강저종류구형성솔화세포침습솔；정농도의뢰성。금작이황소（5、10화20μM）부육간암간세포양세포24 h하조Gli1화Snail1단백표체。결론：금작이황소가능통과조공Gli1억제Snail1단백표체억제간암간세포양세포침습。
Objective To investigate whether and how genistein suppresses the invasion of hepatic cancer stem-like cells(HCSLCs) from MHCC97H cell line.Methods Sphere-forming cells(SFCs) derived from MH-CC97 H cell line which is also called HCSLCs were obtained though suspended culture with stem cell -conditioned medium.After treatment with various concentrations of genistein , the capability of invasion was tested using Tran-swell invasion assay .And the protein expression of Gli 1 and Snail1 was analyzed by western blot .Results MH-CC97 H cells can form tumor spheres though suspended culture with stem cell-conditioned medium .After treatment with genistein(5, 10 and 20 μM) for 72h, the rate of sphere-forming and invasion was decreased in SFCs namely HCSLCs, in a concentration-dependent manner .And the protein expression of Gli1 and Snail1 was down-regulated in HCSLCs.Conclusion Effects of genistein on inhibiting invasion of HCSLCs is likely involved in the reduced protein expression of Snail1 by regulating Gli1.