CAJ | 학술논문

文章研究了简便高效获取毕赤酵母表达系统PCR鉴定模板DNA的提取方法，采用酶消化和物理冻熔结合的方法，提取的酵母基因组DNA直接PCR扩增检测质量。结果表明，该方法提取酵母基因组DNA具有质量高、成本低、耗时短、操作简单等优点。
문장연구료간편고효획취필적효모표체계통PCR감정모판DNA적제취방법，채용매소화화물리동용결합적방법，제취적효모기인조DNA직접PCR확증검측질량。결과표명，해방법제취효모기인조DNA구유질량고、성본저、모시단、조작간단등우점。
In order to obtain a simple and effective method for rapid preparing DNA template to identify Pichia pastoris expression system by PCR amplification, The method of enzyme digestion was combined application with Physics of Freezing & Thawing to extract Pichia pastoris genome DNA, which was detected by PCR as template. The results showed that this method presented advantages of high quality extracts, lower cost, less time consuming and ease of operation.