国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2015年
1期
62-63
,共2页
化学发光微粒子免疫分析%梅毒%诊断
化學髮光微粒子免疫分析%梅毒%診斷
화학발광미입자면역분석%매독%진단
chemiluminescent microparticle immunoassay%syphilis%diagnosis
目的：评估化学发光微粒子免疫分析法（CMIA）在梅毒诊断中的临床价值。方法选择该院确诊的梅毒患者血清标本150例为观察组，另选同期150例非梅毒健康人血清标本为对照组，分别采用甲苯胺红不加热血清试验法（TRUST）、梅毒螺旋体颗粒凝集试验法（TPPA）及 CMIA 法进行检测，计算3种方法的敏感性、准确性及特异性，并对比分析其差异。结果TRUST、TPPA 及 CMIA 3种方法的敏感性分别为65．3％、97．7％及99．3％，特异性为74．7％、97．3％及100．0％，准确性为70．0％、97．0％及99．7％，3种方法之间差异均有统计学意义（P ＞0．05）。TRUST 法敏感性、特异性及准确性均低于 CMIA 法及TPPA 法（P ＞0．05），CMIA 法与 TPPA 法之间差异无统计学意义（P ＞0．05）。结论 CMIA 法敏感性、特异性及准确性与 TPPA法相当，优于 TRUST 法，且具有操作简便、结果判断客观、重复性好等特点。
目的：評估化學髮光微粒子免疫分析法（CMIA）在梅毒診斷中的臨床價值。方法選擇該院確診的梅毒患者血清標本150例為觀察組，另選同期150例非梅毒健康人血清標本為對照組，分彆採用甲苯胺紅不加熱血清試驗法（TRUST）、梅毒螺鏇體顆粒凝集試驗法（TPPA）及 CMIA 法進行檢測，計算3種方法的敏感性、準確性及特異性，併對比分析其差異。結果TRUST、TPPA 及 CMIA 3種方法的敏感性分彆為65．3％、97．7％及99．3％，特異性為74．7％、97．3％及100．0％，準確性為70．0％、97．0％及99．7％，3種方法之間差異均有統計學意義（P ＞0．05）。TRUST 法敏感性、特異性及準確性均低于 CMIA 法及TPPA 法（P ＞0．05），CMIA 法與 TPPA 法之間差異無統計學意義（P ＞0．05）。結論 CMIA 法敏感性、特異性及準確性與 TPPA法相噹，優于 TRUST 法，且具有操作簡便、結果判斷客觀、重複性好等特點。
목적：평고화학발광미입자면역분석법（CMIA）재매독진단중적림상개치。방법선택해원학진적매독환자혈청표본150례위관찰조，령선동기150례비매독건강인혈청표본위대조조，분별채용갑분알홍불가열혈청시험법（TRUST）、매독라선체과립응집시험법（TPPA）급 CMIA 법진행검측，계산3충방법적민감성、준학성급특이성，병대비분석기차이。결과TRUST、TPPA 급 CMIA 3충방법적민감성분별위65．3％、97．7％급99．3％，특이성위74．7％、97．3％급100．0％，준학성위70．0％、97．0％급99．7％，3충방법지간차이균유통계학의의（P ＞0．05）。TRUST 법민감성、특이성급준학성균저우 CMIA 법급TPPA 법（P ＞0．05），CMIA 법여 TPPA 법지간차이무통계학의의（P ＞0．05）。결론 CMIA 법민감성、특이성급준학성여 TPPA법상당，우우 TRUST 법，차구유조작간편、결과판단객관、중복성호등특점。
Objective To evaluate the clinical value of the chemiluminescence immunoassay particles (CMIA)in the diagnosis of syphilis.Methods The serum specimens from 150 cases of syphilis in our hospital were selected as the observation group and con-temporaneous 150 serum samples from non-syphilis healthy people were selected as the control group.The toluidine red unheated serum test (TRUST),treponema pallidum particle agglutination test (TPPA)and CMIA were adopted to conduct the detection. The sensitivity,accuracy and specificity of the three kinds of method were calculated and their differences were compared and ana-lyzed.Results The sensitivity of TRUST and TPPA and CMIA was 65.3%,97.7% and 99.3% respectively,the specificity was 74.7%,97.3% and 100.0% respectively,the accuracy was 70.0%,97.0% and 99.7% respectively,the difference among three kinds of methods had statistical significance (P <0.05).The sensitivity,specificity and accuracy of TRUST were lower than those of CMIA and TPPA (P <0.017),there was no statistically significant difference between CMIA and TPPA (P >0.017).Conclu-sion CMIA is equivalent to TPPA in the sensitivity,specificity and accuracy,is superior to TRUST,and has the advantages of sim-ple operation,objective results and good repeatability.
