CAJ | 학술논문

目的：评估样本收集和保存对全段甲状旁腺素（iPTH）检测结果的影响。方法10份血液透析患者血液样本同时注入4种采血管：促凝管、空白管、EDTA 管及枸橼酸钠管，采用罗氏 cobase411免疫分析仪分别检测在采血后4 h 内，以及4℃保存12、24、48 h 的血清 iPTH 水平。结果采血4 h 内促凝管血清 iPTH 快速发生降解，而空白管中 iPTH 水平最高，且明显高于12、24、48 h。而在 EDTA 管和枸橼酸钠管，iPTH 水平在48 h 才会开始降解。结论首次揭示了促凝剂对血液透析患者血清iPTH 有降解或抑制作用，进一步证实 EDTA 管是 iPTH 最佳的采血管。
목적：평고양본수집화보존대전단갑상방선소（iPTH）검측결과적영향。방법10빈혈액투석환자혈액양본동시주입4충채혈관：촉응관、공백관、EDTA 관급구연산납관，채용라씨 cobase411면역분석의분별검측재채혈후4 h 내，이급4℃보존12、24、48 h 적혈청 iPTH 수평。결과채혈4 h 내촉응관혈청 iPTH 쾌속발생강해，이공백관중 iPTH 수평최고，차명현고우12、24、48 h。이재 EDTA 관화구연산납관，iPTH 수평재48 h 재회개시강해。결론수차게시료촉응제대혈액투석환자혈청iPTH 유강해혹억제작용，진일보증실 EDTA 관시 iPTH 최가적채혈관。
Objective To evaluate the influence of sample collection and storage on the detection results of intact parathyroid hormone(iPTH).Methods 10 blood samples from the hemodialysis patients were simultaneously injected into four types of blood collection tube:pro-coagulation tube,blank tube,EDTA tube and sodium citrate tube.The iPTH levels in the fresh samples within 4 h after blood collection and serum/plasma preserving for 12,24,48 h under 4OC were detected by the Roche cobase411 immunoas-say instrument.Results Serum iPTH in the pro-coagulation tube within 4 h after blood collection was rapidly degraded,while the iPTH level in the blank tube was highest,which was significantly higher than that the serum/plasma preserved for 12,24,48 h.The iPTH level in the EDTA tube and the sodium citrate tube began to degrade only at 48 h.Conclusion It is the first time to reveal that the coagulant has the degrading or inhibiting effect on serum iPTH,which verifies that the EDTA tube is the optimal blood col-lection tube.