河北医科大学学报
河北醫科大學學報
하북의과대학학보
JOURNAL OF HEBEI MEDICAL UNIVERSITY
2015年
1期
42-44
,共3页
韩彬%赵俊莺%何爱萍%舒雅
韓彬%趙俊鶯%何愛萍%舒雅
한빈%조준앵%하애평%서아
右美托咪啶%脂多糖类%肾小管上皮细胞
右美託咪啶%脂多糖類%腎小管上皮細胞
우미탁미정%지다당류%신소관상피세포
dexmedetomidine%lipopolysaccharide%renal tubular epithelial cells
目的:观察右美托咪啶对脂多糖致伤大鼠肾小管上皮细胞Toll样受体4(Toll-like receptor 4,TLR4)表达的影响,并探讨其可能的机制。方法购买大鼠肾小管上皮细胞,经复苏、培养,均加入10μg/L脂多糖,均经培养后分为3组。A组为空白对照组;B组为阳性对照组,加入右美托咪啶2.5μg;C组作为实验组,先加入阿替美唑10μg,30 min后加入右美托咪啶2.5μg。继续培养12 h。采取免疫组织化学法检测3组细胞 TLR4表达及TLR4mRNA水平,并测定3组细胞凋亡率。结果 B组应用右美托咪啶后,TLR4、TLR4mRNA和细胞凋亡率均低于 A组(P<0.01);C组TLR4、TLR4mRNA 和细胞凋亡率均高于 B组(P<0.01)。结论右美托咪啶可下调脂多糖致伤大鼠肾小管上皮细胞TLR4表达,且右美托咪啶对TLR4的调节与α2肾上腺素能受体被激动相关。
目的:觀察右美託咪啶對脂多糖緻傷大鼠腎小管上皮細胞Toll樣受體4(Toll-like receptor 4,TLR4)錶達的影響,併探討其可能的機製。方法購買大鼠腎小管上皮細胞,經複囌、培養,均加入10μg/L脂多糖,均經培養後分為3組。A組為空白對照組;B組為暘性對照組,加入右美託咪啶2.5μg;C組作為實驗組,先加入阿替美唑10μg,30 min後加入右美託咪啶2.5μg。繼續培養12 h。採取免疫組織化學法檢測3組細胞 TLR4錶達及TLR4mRNA水平,併測定3組細胞凋亡率。結果 B組應用右美託咪啶後,TLR4、TLR4mRNA和細胞凋亡率均低于 A組(P<0.01);C組TLR4、TLR4mRNA 和細胞凋亡率均高于 B組(P<0.01)。結論右美託咪啶可下調脂多糖緻傷大鼠腎小管上皮細胞TLR4錶達,且右美託咪啶對TLR4的調節與α2腎上腺素能受體被激動相關。
목적:관찰우미탁미정대지다당치상대서신소관상피세포Toll양수체4(Toll-like receptor 4,TLR4)표체적영향,병탐토기가능적궤제。방법구매대서신소관상피세포,경복소、배양,균가입10μg/L지다당,균경배양후분위3조。A조위공백대조조;B조위양성대조조,가입우미탁미정2.5μg;C조작위실험조,선가입아체미서10μg,30 min후가입우미탁미정2.5μg。계속배양12 h。채취면역조직화학법검측3조세포 TLR4표체급TLR4mRNA수평,병측정3조세포조망솔。결과 B조응용우미탁미정후,TLR4、TLR4mRNA화세포조망솔균저우 A조(P<0.01);C조TLR4、TLR4mRNA 화세포조망솔균고우 B조(P<0.01)。결론우미탁미정가하조지다당치상대서신소관상피세포TLR4표체,차우미탁미정대TLR4적조절여α2신상선소능수체피격동상관。
Objective To observe the effect of dexmedetomidine for Toll-like receptor 4 (TLR4)in renal tubular epithelial cells on rat injured by lipopolysaccharide (LPS),then to explore the possible mechanism.Methods Rat renal tubular epithelial cells were purchased, recovered,cultured,then added LPS 10μg/L and were cultured and divided into three groups, group A,the blank control group,group B added dexmedetomidine 2.5μg as control group,group C joined atipamezole 10μg and added dexmedetomidine 2.5μg after 30 min as the experimental group,all continued culture for 1 2 h.Then,immunohistochemistry was used to measure TLR4 protein expression and TLR4mRNA in three groups,and the apoptosis rate of three groups were determined.Results After using dexmedetomidine,TLR4,TLR4mRNA and apoptotic rate of group B were lower than those of A group (all P<0.01 ),while TLR4,TLR4mRNA and apoptosis rate of group C were higher than those of group B (all P<0.01 ).Conclusion Dexmedetomidine can downregulate TLR4 expression in renal tubular epithelial cells of the rat inj ured by LPS,and the regulation for TLR4 of dexmedetomidine is related withα2 adrenoglc recptor excited.