CAJ | 학술논문

目的：探讨紫外分光光度法测定秋水仙碱中毒血清，为临床秋水仙碱中毒提供诊疗依据。方法取0．5 mL 血清加提取液（三氯甲烷∶甲醇＝95∶5）4．0 mL。充分震荡萃取，高速离心，取有机层于另一试管中氮气吹干，取0．2 mL 甲醇溶解残渣，混匀，取50 mL 做紫外扫描。结果血清中秋水仙碱最大吸收峰（351±1）nm，浓度在5．0～40μg／mL 范围内呈线性，回归方程Y ＝0．0502X ＋0．0013，其相关系数为0．9995，回收率为83．8％～102．8％，相对标准差为3．3％～4．8％。日内、日间相对标准差分别为3．22％～4．74％和3．45％～4．66％，最低检出浓度为1．0μg／mL。结论该方法操作简便，分析快速，结果准确，为临床诊断秋水仙碱中毒提供了一个简便准确的检测方法。
목적：탐토자외분광광도법측정추수선감중독혈청，위림상추수선감중독제공진료의거。방법취0．5 mL 혈청가제취액（삼록갑완∶갑순＝95∶5）4．0 mL。충분진탕췌취，고속리심，취유궤층우령일시관중담기취간，취0．2 mL 갑순용해잔사，혼균，취50 mL 주자외소묘。결과혈청중추수선감최대흡수봉（351±1）nm，농도재5．0～40μg／mL 범위내정선성，회귀방정Y ＝0．0502X ＋0．0013，기상관계수위0．9995，회수솔위83．8％～102．8％，상대표준차위3．3％～4．8％。일내、일간상대표준차분별위3．22％～4．74％화3．45％～4．66％，최저검출농도위1．0μg／mL。결론해방법조작간편，분석쾌속，결과준학，위림상진단추수선감중독제공료일개간편준학적검측방법。
Objective To explore the application of the ultraviolet spectrophotometry method in detecting colchicine poisoning serum to provide the basis for clinical diagnosis and treatment of colchicine poisoning.Methods 0.5 mL of serum was taken and added with the extract solution(chloroform∶methanol=95 ∶5)4.0 mL.The sufficient oscillation extraction and high-speed cen-trifugation were performed.Then the organic layer was taken into another test tube for drying by nitrogen.0.2 mL of methanol dis-solved residue was taken and blended.50 μL of the mixed solution was taken for conducting ultraviolet scanning.Results The max-imum absorption peak of serum colchicine was (351 ±1)nm,the concentration within 5.0-40 μg/mL showed linearity,the regres-sion equation wasY =0.050 2X +0.001 3,the correlation coefficient was 0.999 5,the recovery rate was 83.8% -102.8%,the rel-ative standard deviation:3.3% -4.8%.The intra-day and inter-day were 3.22%-4.74% and 3.45%-4.66%,the lowest detec-tion concentration was 1 .0 μg/mL.Conclusion This method is simple to operate,fast in analysis,accurate in the detection result, which provides a simple and accurate detection method for clinical diagnosis of colchicine poisoning.