CAJ | 학술논문

目的：应用流式细胞仪检测血小板血管扩张剂刺激磷蛋白（VASP）的磷酸化程度，评估 PCI 术后氯吡格雷的应用疗效。方法17例健康对照组不予任何药物干预，26例急性冠脉综合征患者在 PCI 术后给予氯吡格雷抗血小板治疗。分别在入选时和抗血小板治疗后第7天应用流式细胞仪检测血小板 VASP 磷酸化程度，计算血小板反应性指数（PRI）。结果急性冠脉综合征患者在 PRI 术后给予抗血小板治疗的第7天，PRI 明显降低，治疗前后差异有统计学意义（P ＜0．05）。结论流式细胞术检测血小板 VASP 磷酸化程度可以特异性评价氯吡格雷疗效。
목적：응용류식세포의검측혈소판혈관확장제자격린단백（VASP）적린산화정도，평고 PCI 술후록필격뢰적응용료효。방법17례건강대조조불여임하약물간예，26례급성관맥종합정환자재 PCI 술후급여록필격뢰항혈소판치료。분별재입선시화항혈소판치료후제7천응용류식세포의검측혈소판 VASP 린산화정도，계산혈소판반응성지수（PRI）。결과급성관맥종합정환자재 PRI 술후급여항혈소판치료적제7천，PRI 명현강저，치료전후차이유통계학의의（P ＜0．05）。결론류식세포술검측혈소판 VASP 린산화정도가이특이성평개록필격뢰료효。
Objective To use the flow cytometry to detect the platelet vasodilator-stimulated phosphoprotein(VASP)phospho-rylation level and to evaluate the clopidogrel curative effect after PCI.Methods 17 cases in the control group without any drug in-tervention and 26 cases of acute coronary syndrome(ACS)after PCI operation with clopidogrel were selected.Platelet VASP phos-phorylation levels at being selecting and on 7 d after anti-platelet therapy were detected by the flow cytometry and the platelet reac-tivity index (PRI)was calculated.Results The PRI after anti-platelet therapy in the ACS group was decreased significantly,the difference between before treatment and after treatment had statistical significance (P <0.05 ).Conclusion The platelet VASP phosphorylation level detected by the flow cytometry can specifically evaluate the effect of clopidogrel.