国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2015年
1期
12-14
,共3页
曹向红%彭传梅%高辉%付晓野%董玉琳%王杨
曹嚮紅%彭傳梅%高輝%付曉野%董玉琳%王楊
조향홍%팽전매%고휘%부효야%동옥림%왕양
类风湿关节炎%T 细胞免疫球蛋白域黏蛋白域蛋白-1%基因多态性%遗传易感性
類風濕關節炎%T 細胞免疫毬蛋白域黏蛋白域蛋白-1%基因多態性%遺傳易感性
류풍습관절염%T 세포면역구단백역점단백역단백-1%기인다태성%유전역감성
rheumatoid arthritis%T cell immunoglobulin domain and mucin domain protein-1%gene polymorphism%sus-ceptibility
目的:探讨 T 细胞免疫球蛋白域黏蛋白域蛋白(Tim)-1外显子4上的5509-5511delCAA 基因多态性与云南高原地区汉族人群类风湿关节炎(RA)的遗传易感性有无关联。方法采用 PCR-DNA 测序法对196例云南汉族 RA 患者和190例健康体检者 Tim-1外显子4上的5509-5511delCAA 基因多态性进行检测,同时采用 ELISA 法和间接免疫荧光法检测其类风湿因子(RF)、抗环瓜氨酸肽(CCP)、抗角蛋白抗体(AKA)3种自身抗体。结果 RA 组和对照组 Tim-1外显子4上多态性位点5509-5511delCAA 的基因型及等位基因频率差异有统计学意义(P <0.05);RA 组 Tim-1外显子4上5509-5511delCAA 位点不同基因型中3种自身抗体,RF、CCP 阳性率检测的差异无统计学意义(P >0.05),而 AKA 阳性率检测的差异有统计学意义(P <0.05)。结论云南汉族人群 Tim-1外显子4上的5509-5511delCAA 位点存在多态性变异,且其多态性变异与 RA 遗传易感性相关,其不同基因型不会影响 RA 相关自身抗体 RF、CCP 的表达,而对 AKA 的表达有影响。
目的:探討 T 細胞免疫毬蛋白域黏蛋白域蛋白(Tim)-1外顯子4上的5509-5511delCAA 基因多態性與雲南高原地區漢族人群類風濕關節炎(RA)的遺傳易感性有無關聯。方法採用 PCR-DNA 測序法對196例雲南漢族 RA 患者和190例健康體檢者 Tim-1外顯子4上的5509-5511delCAA 基因多態性進行檢測,同時採用 ELISA 法和間接免疫熒光法檢測其類風濕因子(RF)、抗環瓜氨痠肽(CCP)、抗角蛋白抗體(AKA)3種自身抗體。結果 RA 組和對照組 Tim-1外顯子4上多態性位點5509-5511delCAA 的基因型及等位基因頻率差異有統計學意義(P <0.05);RA 組 Tim-1外顯子4上5509-5511delCAA 位點不同基因型中3種自身抗體,RF、CCP 暘性率檢測的差異無統計學意義(P >0.05),而 AKA 暘性率檢測的差異有統計學意義(P <0.05)。結論雲南漢族人群 Tim-1外顯子4上的5509-5511delCAA 位點存在多態性變異,且其多態性變異與 RA 遺傳易感性相關,其不同基因型不會影響 RA 相關自身抗體 RF、CCP 的錶達,而對 AKA 的錶達有影響。
목적:탐토 T 세포면역구단백역점단백역단백(Tim)-1외현자4상적5509-5511delCAA 기인다태성여운남고원지구한족인군류풍습관절염(RA)적유전역감성유무관련。방법채용 PCR-DNA 측서법대196례운남한족 RA 환자화190례건강체검자 Tim-1외현자4상적5509-5511delCAA 기인다태성진행검측,동시채용 ELISA 법화간접면역형광법검측기류풍습인자(RF)、항배과안산태(CCP)、항각단백항체(AKA)3충자신항체。결과 RA 조화대조조 Tim-1외현자4상다태성위점5509-5511delCAA 적기인형급등위기인빈솔차이유통계학의의(P <0.05);RA 조 Tim-1외현자4상5509-5511delCAA 위점불동기인형중3충자신항체,RF、CCP 양성솔검측적차이무통계학의의(P >0.05),이 AKA 양성솔검측적차이유통계학의의(P <0.05)。결론운남한족인군 Tim-1외현자4상적5509-5511delCAA 위점존재다태성변이,차기다태성변이여 RA 유전역감성상관,기불동기인형불회영향 RA 상관자신항체 RF、CCP 적표체,이대 AKA 적표체유영향。
Objective To analyze the association between the polymorphism of 5509-5511delCAA in exon4 of Tim-1 gene and the susceptibility to rheumatoid arthritis(RA)in a group of Han population from Yunnan province.Methods The PCR-DNA se-quencing analysis was used to detect the polymorphism of 5509-5511delCAA in exon4 of Tim-1 gene in 196 RA Han nationality pa-tients and 190 individuals of healthy physical examination(control).The ELISA method and indirect immunofluorescence assay was adopted to detect the three related aotuantibodies of RA,including the rheumatoid factors(RF)and the anti-cyclic citrullinated pep-tide(Anti-CCP)and the anti-keratin antibodies(AKA).Results There were statistically significant differences in genotype and al-lele frequency of 5509-5511delCAA between the RA group and the control group(P <0.05);the positive rate of RF and the anti-CCP between different genotypes of 5509-5511delCAA in the RA group had no statistically significant differences(P >0.05 ),but the positive rate of AKA between different genotypes of 5509-5511delCAA in the RA group had statistically significant differences (P <0.05).Conclusion The 5509-5511delCAA site of Tim-1 exon4 has the polymorphic variation,the 5509-5511delCAA genotype maybe related with the genetic susceptibility to RA in Han population of Yunnan province,the different genotypes does not affect the expression of RF and the anti-CCP,but affects the expression of AKA.