湖泊科学
湖泊科學
호박과학
JOURNAL OF LAKE SCIENCES
2015年
1期
119-127
,共9页
刘丹%白画画%朱元荣%林樱%吴丰昌
劉丹%白畫畫%硃元榮%林櫻%吳豐昌
류단%백화화%주원영%림앵%오봉창
太湖%典型植物%氨基酸
太湖%典型植物%氨基痠
태호%전형식물%안기산
Lake Taihu%typical plants%amino acids
在太湖两个不同湖区(东太湖和贡湖)各选择了8种不同类型的典型水生植物和1种陆生植物全株作为研究对象,采用邻苯二甲醛柱前衍生反相高效液相色谱法对其中15种氨基酸的组成特征进行了分析,并探讨了植物来源氨基酸对湖泊水环境的影响.结果表明东太湖区域植物体中总可水解氨基酸( THAAs)的平均含量为861.6±182.96μmol/g,贡湖区域植物体中THAAs平均含量为700.0±232.3μmol/g;不同类型植物体中 THAAs的含量大小依次为:沉水植物、浮叶植物>挺水植物>陆生植物;其中天冬氨酸、谷氨酸、精氨酸、丙氨酸和赖氨酸是 THAAs的主要组成部分,这5种氨基酸的摩尔浓度占氨基酸总量的50%以上;太湖植物中THAAs所含的氮元素对植物总氮的贡献在30.7%~94.7%之间,是植物体氮元素的主要组分,也是内源氮输入的主要来源.东太湖区域采集的植物样品中各氨基酸的浓度比例与东太湖水体氨基酸组成差异较大,但与沉积物氨基酸组成较吻合,表明东太湖植物来源的有机质和氨基酸是沉积物中有机质和氨基酸的重要来源.
在太湖兩箇不同湖區(東太湖和貢湖)各選擇瞭8種不同類型的典型水生植物和1種陸生植物全株作為研究對象,採用鄰苯二甲醛柱前衍生反相高效液相色譜法對其中15種氨基痠的組成特徵進行瞭分析,併探討瞭植物來源氨基痠對湖泊水環境的影響.結果錶明東太湖區域植物體中總可水解氨基痠( THAAs)的平均含量為861.6±182.96μmol/g,貢湖區域植物體中THAAs平均含量為700.0±232.3μmol/g;不同類型植物體中 THAAs的含量大小依次為:沉水植物、浮葉植物>挺水植物>陸生植物;其中天鼕氨痠、穀氨痠、精氨痠、丙氨痠和賴氨痠是 THAAs的主要組成部分,這5種氨基痠的摩爾濃度佔氨基痠總量的50%以上;太湖植物中THAAs所含的氮元素對植物總氮的貢獻在30.7%~94.7%之間,是植物體氮元素的主要組分,也是內源氮輸入的主要來源.東太湖區域採集的植物樣品中各氨基痠的濃度比例與東太湖水體氨基痠組成差異較大,但與沉積物氨基痠組成較吻閤,錶明東太湖植物來源的有機質和氨基痠是沉積物中有機質和氨基痠的重要來源.
재태호량개불동호구(동태호화공호)각선택료8충불동류형적전형수생식물화1충륙생식물전주작위연구대상,채용린분이갑철주전연생반상고효액상색보법대기중15충안기산적조성특정진행료분석,병탐토료식물래원안기산대호박수배경적영향.결과표명동태호구역식물체중총가수해안기산( THAAs)적평균함량위861.6±182.96μmol/g,공호구역식물체중THAAs평균함량위700.0±232.3μmol/g;불동류형식물체중 THAAs적함량대소의차위:침수식물、부협식물>정수식물>륙생식물;기중천동안산、곡안산、정안산、병안산화뢰안산시 THAAs적주요조성부분,저5충안기산적마이농도점안기산총량적50%이상;태호식물중THAAs소함적담원소대식물총담적공헌재30.7%~94.7%지간,시식물체담원소적주요조분,야시내원담수입적주요래원.동태호구역채집적식물양품중각안기산적농도비례여동태호수체안기산조성차이교대,단여침적물안기산조성교문합,표명동태호식물래원적유궤질화안기산시침적물중유궤질화안기산적중요래원.
The concentrations of total hydrolyzable amino acids( THAAs) were investigated in 8 kinds of typical plants of both East Lake Taihu and Gonghu Bay, in order to find the links and differences of composition of THAAs in the typical plants , sediments and water as well as the composition of THAAs in different typical plants of Lake Taihu. The form and composition of15 kinds of THAAs in 18 samples were analyzed by o-phthalaldehyde pre-column derivatization-RP-HPLC.The average concentration of THAAs in typical plants of East Lake Taihu was 861.6 ±182.96μmol/g.The average concentration in typical plants of Gonghu Bay was 700.0 ±232.3 μmol/g.The comparison of the concentrations of THAAs in different kinds of plants showed submerged macrophytes and floating-leaved macrophytes had the highest concentration of THAAs, followed by emerged macrophytes and terres-trial plants.The dominating amino acids were Asparagine, Glutamic acid, Arginine, Alanine and Lysine, which accounted for more than 50% of concentration of THAAs.About 30.7% 94.7% of the N in plants were from THAAs, which was an important source of N in plants.Meanwhile, in comparison with the composition of THAAs in sediments and water in Lake Taihu , the typical plants generally aligned with sediments but different from the water .It showed that THAAs in plant may be an important source of the THAAS in sediments.