CAJ | 학술논문

目的：用酶联免疫吸附试验（ELISA）法检测乙型肝炎病毒（HBV）标志物，若乙型肝炎病毒核心抗体（HBcAb）单项或 HBcAb 与乙型肝炎病毒 e 抗体（HBeAb）二项阳性，再进行乙型肝炎病毒表面抗原（HBsAg）定量和 HBV 载量测定，了解 HBV携带及病毒复制情况。方法 ELISA 检测 HBV 标志物 HBsAg、HBsAb、HBeAg、HBeAb、HBcAb，选取1098例 HBcAb 阳性、966例 HBeAb 和 HBcAb 二项阳性样本及832例 HBV 标志物全阴性标本为对照，用化学发光法定量复检 HBsAg，并用 PCR 的方法检测 HBV 载量。结果1098例 HBcAb 单项阳性检测出 HBsAg 定量436例（39．7％）、PCR-DNA230例（20．9％）；966例HBeAb、HBcAb 二项阳性的标本分别定量检测出 HBsAg 定量387例（40．1％）、PCR-DNA 212例（21．9％），HBV 标志物全阴性的 HBsAg 定量和 PCR-DNA 比较，差异有统计学意义（P ＜0．05）。结论 ELISA 法检测 HBV 标志物时，若只 HBcAb 阳性或HBcAb 与 HBeAb 二项阳性，仍可检出表面抗原和病毒的复制，需做进一步的检测，以免漏检，造成医疗风险。
목적：용매련면역흡부시험（ELISA）법검측을형간염병독（HBV）표지물，약을형간염병독핵심항체（HBcAb）단항혹 HBcAb 여을형간염병독 e 항체（HBeAb）이항양성，재진행을형간염병독표면항원（HBsAg）정량화 HBV 재량측정，료해 HBV휴대급병독복제정황。방법 ELISA 검측 HBV 표지물 HBsAg、HBsAb、HBeAg、HBeAb、HBcAb，선취1098례 HBcAb 양성、966례 HBeAb 화 HBcAb 이항양성양본급832례 HBV 표지물전음성표본위대조，용화학발광법정량복검 HBsAg，병용 PCR 적방법검측 HBV 재량。결과1098례 HBcAb 단항양성검측출 HBsAg 정량436례（39．7％）、PCR-DNA230례（20．9％）；966례HBeAb、HBcAb 이항양성적표본분별정량검측출 HBsAg 정량387례（40．1％）、PCR-DNA 212례（21．9％），HBV 표지물전음성적 HBsAg 정량화 PCR-DNA 비교，차이유통계학의의（P ＜0．05）。결론 ELISA 법검측 HBV 표지물시，약지 HBcAb 양성혹HBcAb 여 HBeAb 이항양성，잉가검출표면항원화병독적복제，수주진일보적검측，이면루검，조성의료풍험。
Objective To use the enzyme linked immunosorbent (ELISA)to detect the hepatitis B virus (HBV)markers,and to perform the HBsAg quantitation and the HBV load detection for understanding the HBV carrying and viral replication situation when single HBcAb positive or both HBcAb and HBeAb positive.Methods The HBV markers HBsAg,HBsAb,HBeAg,HBeAb and HBcAb were detected with ELISA.1 098 cases of HBcAb positive,966 cases of both HBeAb and HBcAb positive and 832 cases of all HBV markers negative as control were selected and quantitatively re-detected HBsAg by using the chemiluminescence meth-od.The HBV load was detected by using the PCR method.Results Among 1 098 cases of single HBcAb positive,436 cases (39.7%)of HBsAg quantitation and 230 cases (20.9%)of PCR-DNA were detected out respectively;among 966 cases of both HBeAb and HBcAb positive,387 cases(40.1 %)of HBsAg quantitation and 212 cases(21.9%)of PCR-DNA were detected out re-spectively;among 832 cases of all HBV markers negative,6 case (0.7%)of HBsAg quantitation and 4 case (0.5%)of PCR-DNA were detected out respectively,there were statistically significantly differences among them (P < 0.05 ).Conclusion Adopting ELISA for detecting HBV markers,when single HBeAb positive or both HBcAb and HBeAb positive,HBsAg and the viral replica-tion are still be detected out,which needs to conduct further detection in order to avoid the medical risk due to the missed detection.