CAJ | 학술논문

以鱼诺卡氏菌(Nocardia seriolae)为研究材料，经扩大培养，离心集菌，用三氯乙酸处理以除去细胞壁上的磷壁酸及菌体内的核酸，甲醇、丙酮脱脂后，再经胰蛋白酶及中性蛋白酶复合酶解去除菌体内部的蛋白质，离心清洗、冻干即可制得肽聚糖。经过溶菌酶溶解试验，紫外可见光谱扫描分析证明所得产物为肽聚糖，透射电镜观察显示所提取的肽聚糖为囊状结构，且仍保持着原有的细菌细胞形态，证实所提取的肽聚糖为全肽聚糖。
이어낙잡씨균(Nocardia seriolae)위연구재료，경확대배양，리심집균，용삼록을산처리이제거세포벽상적린벽산급균체내적핵산，갑순、병동탈지후，재경이단백매급중성단백매복합매해거제균체내부적단백질，리심청세、동간즉가제득태취당。경과용균매용해시험，자외가견광보소묘분석증명소득산물위태취당，투사전경관찰현시소제취적태취당위낭상결구，차잉보지착원유적세균세포형태，증실소제취적태취당위전태취당。
The cell wall WPG of Nocardia seriolae was extracted and purified as follows: bacteria were collected by centrifuge after culture expansion. The bacteria cells were treated with trichloroacetic acid in order to remove the wall teichoic acids as well as nucleic acid. The residues were degreased with methanol and acetone. Then we digested the defatted residues with trypsin and neutral protease. They were cleaned by centrifugation and lyophilization. It has been proved by lysozyme and UV-visible absorption spectra that the extracts were peptidoglycan. The transmission electeonmicroscopic observation of WPG showed that WPG was in sacciform and still held the physically intact cell wall structure of the whole cells, which certified the peptidoglycan we obtained was whole peptidoglycan.