CAJ | 학술논문

以宋河酒厂窖泥、酒糟、酒厂废水为样品，从中分离出100株菌株，经初筛、复筛和酒精发酵试验，最终获得1株耐酒精度为18%vol酵母菌，编号为A16。通过单因素实验确定最佳发酵条件为：发酵温度30℃、pH值4.5、碳源为葡萄糖、氮源为复合氮源（酵母膏和蛋白胨的比例为2∶1）、无机盐为MgSO4·7H2O、接种量为10%、转速100 r/min、装液量为150 mL/250 mL；通过正交试验确定培养基最佳配比为：葡萄糖25%，酵母膏2%，蛋白胨1%， MgSO4·7H2O 1%，（NH4）2SO41%，KH2PO41%，菌株A16的酒精得率70%。
이송하주엄교니、주조、주엄폐수위양품，종중분리출100주균주，경초사、복사화주정발효시험，최종획득1주내주정도위18%vol효모균，편호위A16。통과단인소실험학정최가발효조건위：발효온도30℃、pH치4.5、탄원위포도당、담원위복합담원（효모고화단백동적비례위2∶1）、무궤염위MgSO4·7H2O、접충량위10%、전속100 r/min、장액량위150 mL/250 mL；통과정교시험학정배양기최가배비위：포도당25%，효모고2%，단백동1%， MgSO4·7H2O 1%，（NH4）2SO41%，KH2PO41%，균주A16적주정득솔70%。
In this study, 100 yeast strains were isolated from pit mud, distillers' grains, and waste water in Songhe Distillery. After primary screening, secondary screening, and ethanol fermenting test, an ethanol-tolerance yeast strain named A16 (tolerating 18%vol alcohol content) was finally obtained. The optimum fermentation conditions of A16 were summed up as follows by single factor test:fermenting temperature at 30℃, initial pH value was 4.5,carbon sources composed of glucose, nitrogen source was complex nitrogen source (the ratio of yeast extract and peptone was 2∶1), inorganic salts was MgSO4·7H2O, the inoculating amount was 10%, the rotating speed was 100 r/min, and liquid-filling vol-ume was 150 mL/250 mL. Besides, the best proportioning of culture mediums for A16 was determined through orthogonal test as follows:25%glucose, 2%yeast extract, 1%peptone, 1%MgSO4·7H2O, 1%(NH4)2SO4, and 1% KH2PO4. As a result, ethanol yield of strain A16 was 70%.