中华糖尿病杂志
中華糖尿病雜誌
중화당뇨병잡지
CHINES JOURNAL OF DLABETES MELLITUS
2015年
2期
115-119
,共5页
糖尿病肾病%β2糖蛋白1%转化生长因子-β1%BALB/c小鼠
糖尿病腎病%β2糖蛋白1%轉化生長因子-β1%BALB/c小鼠
당뇨병신병%β2당단백1%전화생장인자-β1%BALB/c소서
Diabetic nephropathy%β2- Glycoprotein I%Transforming growth factor β1%BALB/c mice
目的:观察不同时长β2糖蛋白1(β2-GP1)对链脲佐菌素(STZ)诱导的糖尿病鼠肾组织病理学改变及转化生长因子β1(TGF-β1)表达水平的影响。方法以48只雌性BALB/c小鼠为对象,持续喂养8周高糖高脂饮食,后腹腔注射STZ(80 mg/kg),1周后以随机血糖≥16.7 mmol/L视为成功建立糖尿病小鼠模型。采用随机数字表法将成模糖尿病小鼠分为模型组(12只)和β2-GP1干预组(20μgβ2-GP1干预3周及6周组,各12只),并设正常对照组(12只),干预3周及6周后检测肾组织TGF-β1分布及肾皮质TGF-β1 mRNA及蛋白表达水平。组间比较采用单因素方差分析,进行方差齐性检验,组间两两比较采用SNK-q检验。结果β2-GP1干预组能够明显减低肾皮质TGF-β1 mRNA表达水平(3.14±0.14、1.98±0.11比6.07±0.22,q=18.037、28.454,均P<0.05)及蛋白表达水平(0.66±0.07、0.40±0.05比1.44±0.08,q=12.757、16.454,均P<0.05)。结论β2-GP1可抑制STZ诱导的糖尿病小鼠肾脏TGF-β1的表达,并具有一定的时间依赖性,该作用可能与其肾脏保护作用有关。
目的:觀察不同時長β2糖蛋白1(β2-GP1)對鏈脲佐菌素(STZ)誘導的糖尿病鼠腎組織病理學改變及轉化生長因子β1(TGF-β1)錶達水平的影響。方法以48隻雌性BALB/c小鼠為對象,持續餵養8週高糖高脂飲食,後腹腔註射STZ(80 mg/kg),1週後以隨機血糖≥16.7 mmol/L視為成功建立糖尿病小鼠模型。採用隨機數字錶法將成模糖尿病小鼠分為模型組(12隻)和β2-GP1榦預組(20μgβ2-GP1榦預3週及6週組,各12隻),併設正常對照組(12隻),榦預3週及6週後檢測腎組織TGF-β1分佈及腎皮質TGF-β1 mRNA及蛋白錶達水平。組間比較採用單因素方差分析,進行方差齊性檢驗,組間兩兩比較採用SNK-q檢驗。結果β2-GP1榦預組能夠明顯減低腎皮質TGF-β1 mRNA錶達水平(3.14±0.14、1.98±0.11比6.07±0.22,q=18.037、28.454,均P<0.05)及蛋白錶達水平(0.66±0.07、0.40±0.05比1.44±0.08,q=12.757、16.454,均P<0.05)。結論β2-GP1可抑製STZ誘導的糖尿病小鼠腎髒TGF-β1的錶達,併具有一定的時間依賴性,該作用可能與其腎髒保護作用有關。
목적:관찰불동시장β2당단백1(β2-GP1)대련뇨좌균소(STZ)유도적당뇨병서신조직병이학개변급전화생장인자β1(TGF-β1)표체수평적영향。방법이48지자성BALB/c소서위대상,지속위양8주고당고지음식,후복강주사STZ(80 mg/kg),1주후이수궤혈당≥16.7 mmol/L시위성공건립당뇨병소서모형。채용수궤수자표법장성모당뇨병소서분위모형조(12지)화β2-GP1간예조(20μgβ2-GP1간예3주급6주조,각12지),병설정상대조조(12지),간예3주급6주후검측신조직TGF-β1분포급신피질TGF-β1 mRNA급단백표체수평。조간비교채용단인소방차분석,진행방차제성검험,조간량량비교채용SNK-q검험。결과β2-GP1간예조능구명현감저신피질TGF-β1 mRNA표체수평(3.14±0.14、1.98±0.11비6.07±0.22,q=18.037、28.454,균P<0.05)급단백표체수평(0.66±0.07、0.40±0.05비1.44±0.08,q=12.757、16.454,균P<0.05)。결론β2-GP1가억제STZ유도적당뇨병소서신장TGF-β1적표체,병구유일정적시간의뢰성,해작용가능여기신장보호작용유관。
Objective To explore the effects of β2-glycoprotein I(β2-GP1)on renal tissue pathologic change and transforming growth factor β1(TGF-β1)expression in streptozotocin(STZ)induced diabetic mice. Methods Forty-eight female BALB/c mice were fed with high fat chow diet for 8 weeks. Then the mice were injected intraperitoneally with STZ(80 mg/kg). Diabetic mice were confirmed by the criterion that plasma glucose concentration was higher than 16.7 mmol/L after 1 week from injection date. The mice were randomly divided into a diabetes mellitus(DM)group(n=12),treatment withβ2-GP1(20μg) groups(treated for 3 weeks or 6 weeks,each n=12)and a normal control group(n=12). The changes of glomerular structure and expression of TGF-β1 in renal cortical were detected by immunohistochemical techniques,quantitative real-time PCR and Western blotting. Single factor analysis of variance(ANOVA) was applied to perform the homogeneity of variance test among the groups,and SNK-q test were used to analyze the difference between two groups. Results β2-GP1 improved renal dysfunction and kidney structure damage,and decreased TGF-β1 mRNA expression(3.14±0.14,1.98±0.11 vs 6.07±0.22,q=18.037, 28.454,respectively,all P<0.05)and protein expression(0.66 ± 0.07,0.40 ± 0.05 vs 1.44 ± 0.08,q=12.757, 16.454,respectively,all P<0.05)in STZ-induced diabetic. Conclusion β2-GP1 plays a role of renal protecting by inhibiting the expression of TGF-β1 in renal tissues of the STZ-diabetic mice,may in time-dependent way.