CAJ | 학술논문

真菌产生的淀粉酶具有水解条件温和耐酸的特性，具有广泛的应用价值。为了筛选高产淀粉酶黑曲霉菌种，通过采用微波诱变和硫酸二乙酯处理等方法联合诱变黑曲霉出发菌株，并对筛选出的黑曲霉菌株固态发酵产α-淀粉酶最佳条件进行了优化。结果显示，经过微波诱变16 s和硫酸二乙酯处理20 min筛选出了一株高产α-淀粉酶菌株黑曲霉MD-17，其最佳产酶固态发酵条件为：麸皮与玉米粉干重比为7：3，硫酸铵0.6%，初始pH=5，料水比1：1.5，α-淀粉酶的酶活力最高达到6011 U/g湿曲。
진균산생적정분매구유수해조건온화내산적특성，구유엄범적응용개치。위료사선고산정분매흑곡매균충，통과채용미파유변화류산이을지처리등방법연합유변흑곡매출발균주，병대사선출적흑곡매균주고태발효산α-정분매최가조건진행료우화。결과현시，경과미파유변16 s화류산이을지처리20 min사선출료일주고산α-정분매균주흑곡매MD-17，기최가산매고태발효조건위：부피여옥미분간중비위7：3，류산안0.6%，초시pH=5，료수비1：1.5，α-정분매적매활력최고체도6011 U/g습곡。
The fungal amylase has the characteristic of mild hydrolusis conditions and acid-resistance, and a wide range of application. By composite mutagenesis of microwave mutagenesis and diethyl sulfate treatment, isolate with high yield ofα-amylase was screened and the solid-state fermentation conditions were optimized. The results showed that, A. niger MD-17 was obtained after 16 s microwave mutagenesis and 20 min diethyl sulfate treatment. The optimal solid-state fermentation conditions followed as bran:corn flour=7:3;(NH4)2SO4:0.6%;initial pH 5;solid material:water 1:1.5. Fungalα-amylase enzyme activity could reached the highest 6011 U/g.