CAJ | 학술논문

研究了洋山航道工程疏浚泥悬浮液和浸出液对三疣梭子蟹（ Portunus trituberculatus）大眼幼体的毒害效应，结果表明：（1）大眼幼体死亡率随着悬浮液和浸出液配比浓度的增大而逐渐增加。浸出液对大眼幼体的96 h半致死配比浓度φ（ LC50）为1294?66 mL·L－1，安全配比浓度φ（ SC）为129?466 mL·L－1；悬浮液对大眼幼体的96 hφ（LC50）为996?17 mL·L－1，φ（SC）为99?617 mL·L－1。（2）大眼幼体的蜕皮率随着悬浮液和浸出液配比浓度的增加而逐渐降低。同体积配比浓度下，浸出液处理组蜕皮率略高于悬浮液处理组。悬浮液抑制三疣梭子蟹大眼幼体的蜕壳，而浸出液抑制新壳的硬化。（ 3）浸出液和悬浮液胁迫24 h后，大眼幼体肌肉中HSP70表达量迅速升高，浸出液和悬浮液最高配比浓度组表达量显著高于对照组（ P＜0?05）；72 h各处理组HSP70表达量均达到最高值；96 h后各处理组表达量有所降低，最高配比浓度组HSP70表达量降幅明显（ P＜0?05），对照组表达量仍继续上升。
연구료양산항도공정소준니현부액화침출액대삼우사자해（ Portunus trituberculatus）대안유체적독해효응，결과표명：（1）대안유체사망솔수착현부액화침출액배비농도적증대이축점증가。침출액대대안유체적96 h반치사배비농도φ（ LC50）위1294?66 mL·L－1，안전배비농도φ（ SC）위129?466 mL·L－1；현부액대대안유체적96 hφ（LC50）위996?17 mL·L－1，φ（SC）위99?617 mL·L－1。（2）대안유체적세피솔수착현부액화침출액배비농도적증가이축점강저。동체적배비농도하，침출액처리조세피솔략고우현부액처리조。현부액억제삼우사자해대안유체적세각，이침출액억제신각적경화。（ 3）침출액화현부액협박24 h후，대안유체기육중HSP70표체량신속승고，침출액화현부액최고배비농도조표체량현저고우대조조（ P＜0?05）；72 h각처리조HSP70표체량균체도최고치；96 h후각처리조표체량유소강저，최고배비농도조HSP70표체량강폭명현（ P＜0?05），대조조표체량잉계속상승。
Toxic effect of suspension and lixivium from the dredged sludge of the Yangshan channel project on megalopa of Portunus trituberculatus was evaluated, through a laboratory simulation experiment. Results show that ( 1) mortality of the megalopa increased gradually with concentration of the suspension and lixivium, and 96 h LC50 and SC of the lixivium to megalopa of Portunus trituberculatus was 1 294?66 and 129?466 mL·L-1, respectively, and of the suspension, 996?17 and 99?617 mL·L-1 , respectively;( 2) molting rate of the megalopa decreased with increasing concentration of the sus?pension and lixivium;it was slightly higher in the group of lixivium treatments than in the group of suspension treatments group when they were the same in volume concentration;the suspension inhibited molting of megalopa, while the lixivium inhibited hardening of new shell;( 3) After 24 hours of exposure to the suspension and lixivium, the expression of HSP70 in the muscle of megalopa increased rapidly; after 72 hours, it peaked in all the treatments; and after 96 hours, it de?creased to a varying extent in all the treatments and the most significantly in the treatment the highest in concentration;while in the control it kept on rising.