CAJ | 학술논문

介绍了在生物工程实验中探索利用分组协同创新模式进行实验教学的成果。将一个教学自然班分成12个实验小组，每组同学3人。以可能影响实验结果的四个变量分解成四个独立又互相联系的实验，每个变量分别由三组学生负责研究，研究过程通过定期协调沟通对实验结果进行分析总结并不断优化实验设计，得出了优化实验工艺，同时也培养了学生独立思考和协作配合的精神。实验研究了戊二醛交联糖化酶的优化条件，考察了pH、戊二醛浓度、温度和时间四个变量对交联结果的影响。综合分析各实验小组的实验结果显示：酸性条件交联有利于酶活力及稳定性的保持；使用0.5%或更低的戊二醛浓度有利于酶活力的保持，但其稳定性提升不明显；较高温度下交联不利于酶活力的保持及稳定性的提升；随着交联时间的增加酶活力损失逐渐变大，但其使用稳定性却有所提升。
개소료재생물공정실험중탐색이용분조협동창신모식진행실험교학적성과。장일개교학자연반분성12개실험소조，매조동학3인。이가능영향실험결과적사개변량분해성사개독립우호상련계적실험，매개변량분별유삼조학생부책연구，연구과정통과정기협조구통대실험결과진행분석총결병불단우화실험설계，득출료우화실험공예，동시야배양료학생독립사고화협작배합적정신。실험연구료무이철교련당화매적우화조건，고찰료pH、무이철농도、온도화시간사개변량대교련결과적영향。종합분석각실험소조적실험결과현시：산성조건교련유리우매활력급은정성적보지；사용0.5%혹경저적무이철농도유리우매활력적보지，단기은정성제승불명현；교고온도하교련불리우매활력적보지급은정성적제승；수착교련시간적증가매활력손실축점변대，단기사용은정성각유소제승。
The exploration of cooperative innovation mode of experimental teaching by using packet results in biological engineering experiment was introduced. A natural class was divided into 12 experimental groups for 3 students each group. The four variables which may influence the results were dismantled into four independent and interrelated experiments, for this study, three groups of students were given an experiment. Through regular communication and coordination for the experimental results, and the continuous optimization for the design of experiment, the optimization of experimental process was got, as well as, the spirit for the students to think independently and cooperation was also cultivated. The effects of pH, glutaraldehyde concentration, temperature and cross-linking time were studied in this experiment for optimization of the conditions of glutaraldehyde cross-linked glucoamylase. The results from comprehensive analysis of all the experimental groups showed that acidic conditions was favorable for remain of cross-linking enzyme activity and stability, the glutaraldehyde concentration less than 0. 5% was also favorable to keep the enzyme activity but not obvious for stability, higher temperature was not conducive to the promotion of enzyme activity and stability, and the loss of enzyme activity gradually increased with the cross-linking time, on the other hand, the stability of the cross-linked enzyme exhibited a increase with the time enhance.