广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2015年
2期
72-73,99
,共3页
广陈皮%DNA条形码%研究
廣陳皮%DNA條形碼%研究
엄진피%DNA조형마%연구
Citri Reticulatae Pericarpium from Guangdong%DNA barcode%research
陈皮( Citri Reticulatae Pericarpium )为芸香科植物橘Citrus Reticulata Blanco及其栽培变种的干燥成熟果皮。根据药材来源不同又可分为“陈皮”和“广陈皮”。其中“广陈皮”是变种的茶枝柑果皮，为公认的道地药材。本文拟在分子水平建立广陈皮的DNA条形码，并对来源不同的陈皮样本在trnH-psbA序列进行分析，建立广陈皮的DNA序列。结果表明广陈皮样本与橙类果皮样本之间有明显的差异。因此，判断trnH-psbA序列DNA条形码可用于初步鉴别广陈皮。
陳皮( Citri Reticulatae Pericarpium )為蕓香科植物橘Citrus Reticulata Blanco及其栽培變種的榦燥成熟果皮。根據藥材來源不同又可分為“陳皮”和“廣陳皮”。其中“廣陳皮”是變種的茶枝柑果皮，為公認的道地藥材。本文擬在分子水平建立廣陳皮的DNA條形碼，併對來源不同的陳皮樣本在trnH-psbA序列進行分析，建立廣陳皮的DNA序列。結果錶明廣陳皮樣本與橙類果皮樣本之間有明顯的差異。因此，判斷trnH-psbA序列DNA條形碼可用于初步鑒彆廣陳皮。
진피( Citri Reticulatae Pericarpium )위예향과식물귤Citrus Reticulata Blanco급기재배변충적간조성숙과피。근거약재래원불동우가분위“진피”화“엄진피”。기중“엄진피”시변충적다지감과피，위공인적도지약재。본문의재분자수평건립엄진피적DNA조형마，병대래원불동적진피양본재trnH-psbA서렬진행분석，건립엄진피적DNA서렬。결과표명엄진피양본여등류과피양본지간유명현적차이。인차，판단trnH-psbA서렬DNA조형마가용우초보감별엄진피。
Pericarpium Citri Reticulatae was from the dry and mature skin of Citrus reticulata Blanco and cultivar. According to different sources of medicinal materials, it can be divided into‘orange’ and‘wide dried tangerine or orange peel’ . Citri Reticulatae Pericarpium from Guangdong from‘Chazhi’ was recognized as Chinese drugs. A method on DNA barcode of Citri Reticulatae Pericarpium from Guangdong was established. The trnH-psbA sequences of different samples of Citri Reticulatae Pericarpium were analyzed in order to establish DNA barcode of Citri Reticulatae Pericarpium from Guangdong. So the trnH-psbA DNA barcode can be used to preliminary identification of Citri Reticulatae Pericarpium from Guangdong.
