CAJ | 학술논문

原生质体培养是体细胞杂交的基础，同时也是获得变异材料的重要手段。以引进的4个二倍体马铃薯为材料，研究了渗透浓度、酶液组成、酶解时间及材料预处理对原生质体产量和活力的影响。结果表明：预处理是原生质体成活、分裂及后期发育的重要条件，处理D预处理效果最好，原生质产量和活力最高，且有较多细胞分裂；马铃薯原生质体适宜的渗透浓度为0．35～0．40 mol?L‐1；RH2和RH3酶解时间以4 h为宜，RH1和RH4酶解时间以4．5h为宜；酶解液为2．0％纤维素酶和0．2％果胶酶的混合液。基因型对原生质体培养影响较大。经过培养，有2个二倍体材料获得了再生植株。
원생질체배양시체세포잡교적기출，동시야시획득변이재료적중요수단。이인진적4개이배체마령서위재료，연구료삼투농도、매액조성、매해시간급재료예처리대원생질체산량화활력적영향。결과표명：예처리시원생질체성활、분렬급후기발육적중요조건，처리D예처리효과최호，원생질산량화활력최고，차유교다세포분렬；마령서원생질체괄의적삼투농도위0．35～0．40 mol?L‐1；RH2화RH3매해시간이4 h위의，RH1화RH4매해시간이4．5h위의；매해액위2．0％섬유소매화0．2％과효매적혼합액。기인형대원생질체배양영향교대。경과배양，유2개이배체재료획득료재생식주。
Protoplast culture is the foundation of somatic hybridization and an important method to acquire mu‐tant material .Taking four introduced diploid potato as materials ,the effect of osmotic concentration ,enzymoly‐sis conduction ,enzymolysis time and pretreatment on yield and vitality of protoplast were studied .The results indicated that pretreatment was a key factor for surviving ,division and development of protoplast .The proto‐plast yield and vitality of treatment D were the best .The osmotic concentration suitable for protoplast was 0 .35~0 .40 m?L‐1 .The suitable enzymolysis time for RH2 and RH3 was 4 h ,RH1 and RH4 was 4 .5 h .Enzy‐matic hydrolysate was 2 .0% Cellulase RS Onozuka and 0 .2% Pectolyase Y‐23 .The protoplast culture was greatly influenced by genotypes .Regeneration plantlets of two diploid potato materials were successfully gained .