江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2015年
1期
32-35
,共4页
杨敬辉%陈宏州%肖婷%张文文%庄义庆
楊敬輝%陳宏州%肖婷%張文文%莊義慶
양경휘%진굉주%초정%장문문%장의경
葡萄炭疽病菌%多菌灵%抗药性%检测
葡萄炭疽病菌%多菌靈%抗藥性%檢測
포도탄저병균%다균령%항약성%검측
Grape anthracnose pathogen%Carbendazim%Resistance%Detection
采用菌丝生长速率法和区分剂量法检测了采自江苏省句容市的98株葡萄炭疽病菌对多菌灵的抗药性。菌丝生长速率法检测结果表明:多菌灵抑制葡萄炭疽病菌菌丝生长的EC50值在0.0610~2.2991μg/mL之间,平均为0.5285μg/mL;敏感、中抗和高抗菌株分别占55.10%、43.88%和1.02%。区分剂量法检测结果表明:敏感、低抗、中抗和高抗菌株分别占51.02%、45.92%、3.06%和0%。
採用菌絲生長速率法和區分劑量法檢測瞭採自江囌省句容市的98株葡萄炭疽病菌對多菌靈的抗藥性。菌絲生長速率法檢測結果錶明:多菌靈抑製葡萄炭疽病菌菌絲生長的EC50值在0.0610~2.2991μg/mL之間,平均為0.5285μg/mL;敏感、中抗和高抗菌株分彆佔55.10%、43.88%和1.02%。區分劑量法檢測結果錶明:敏感、低抗、中抗和高抗菌株分彆佔51.02%、45.92%、3.06%和0%。
채용균사생장속솔법화구분제량법검측료채자강소성구용시적98주포도탄저병균대다균령적항약성。균사생장속솔법검측결과표명:다균령억제포도탄저병균균사생장적EC50치재0.0610~2.2991μg/mL지간,평균위0.5285μg/mL;민감、중항화고항균주분별점55.10%、43.88%화1.02%。구분제량법검측결과표명:민감、저항、중항화고항균주분별점51.02%、45.92%、3.06%화0%。
In this study, 98 strains of grape anthracnose pathogen were collected from Jurong city, Jiangsu province, and their resistances to Carbendazim were detected by using mycelial growth rate method and dosage distinguishing method.The result of de-tection by mycelial growth rate method showed that the EC50 values of Carbendazim against the mycelial growth of these strains varied from 0.0610 μg/mL to 2.2991 μg/mL, with an average of 0.5285 μg/mL; the percentage of sensitive, moderately resistant and highly resistant strains was 55.10%, 43.88% and 1.02%, respectively.The result of detection by dosage distinguishing method indicated that the sensitive, lowly resistant, moderately resistant and highly resistant strains accounted for 51.02%, 45.92%, 3. 06%and 0%of total strains respectively.