江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2015年
1期
28-31
,共4页
朱辉%宋薇薇%余凤玉%刘丽%覃伟权
硃輝%宋薇薇%餘鳳玉%劉麗%覃偉權
주휘%송미미%여봉옥%류려%담위권
槟榔%炭疽病%病原菌%鉴定%rDNA-ITS序列
檳榔%炭疽病%病原菌%鑒定%rDNA-ITS序列
빈랑%탄저병%병원균%감정%rDNA-ITS서렬
Arecanut%Anthracnose%Pathogen%Identification%rDNA-ITS sequence
研究了海南槟榔炭疽病的症状及其病原菌的形态特征、致病性,并分析了病原菌的rDNA-ITS序列。结果表明:从海南槟榔的5个主要栽培区采集的26份病样中均获得了相似的分离物;病原菌分生孢子长卵圆形或圆柱形,大小为(12.0~21.3)μm ×(3.5~6.0)μm,经回接后形成的症状与田间典型症状一致;病原菌在系统进化树上与胶孢炭疽菌处在同一分支,同源性达100%。综合该病原菌的形态特征、致病性和rDNA-ITS区段的分析结果,将海南槟榔炭疽病的病原鉴定为胶孢炭疽菌( Colletotrichum gloeosporioides)。
研究瞭海南檳榔炭疽病的癥狀及其病原菌的形態特徵、緻病性,併分析瞭病原菌的rDNA-ITS序列。結果錶明:從海南檳榔的5箇主要栽培區採集的26份病樣中均穫得瞭相似的分離物;病原菌分生孢子長卵圓形或圓柱形,大小為(12.0~21.3)μm ×(3.5~6.0)μm,經迴接後形成的癥狀與田間典型癥狀一緻;病原菌在繫統進化樹上與膠孢炭疽菌處在同一分支,同源性達100%。綜閤該病原菌的形態特徵、緻病性和rDNA-ITS區段的分析結果,將海南檳榔炭疽病的病原鑒定為膠孢炭疽菌( Colletotrichum gloeosporioides)。
연구료해남빈랑탄저병적증상급기병원균적형태특정、치병성,병분석료병원균적rDNA-ITS서렬。결과표명:종해남빈랑적5개주요재배구채집적26빈병양중균획득료상사적분리물;병원균분생포자장란원형혹원주형,대소위(12.0~21.3)μm ×(3.5~6.0)μm,경회접후형성적증상여전간전형증상일치;병원균재계통진화수상여효포탄저균처재동일분지,동원성체100%。종합해병원균적형태특정、치병성화rDNA-ITS구단적분석결과,장해남빈랑탄저병적병원감정위효포탄저균( Colletotrichum gloeosporioides)。
The symptoms of arecanut anthracnose in Hainan as well as the morphological characteristics and pathogenicity of pathogen of this disease were studied, and the ribosomal DNA-ITS sequence of this pathogen was analyzed.The results indicated that similar isolates were all obtained from 26 diseased arecanut samples collected from 5 main cultivating regions of Hainan; the conidia of this pathogen were oval or cylindrical, and their size was (12.0~21.3)μm ×(3.5~6.0)μm;after being inoculated, this pathogen caused the same symptoms as the typical symptoms in field diseased samples; the pathogen was in the same branch with Colletotrichum gloeosporioides in phylogenetic tree, and the homology of this two reached 100%.According to the morphological characteristics, pathogenicity and rDNA-ITS sequence of the pathogen of arecanut anthracnose in Hainan, it was identified as Col-letotrichum gloeosporioides.