CAJ | 학술논문

目的：建立测定复方甘草麻黄碱片中麻黄碱含量的方法。方法采用反相高效液相色谱法，色谱柱为 Diamonsil (钻石) C18柱(250 mm ×4.6 mm，5μm），流动相为0.1 mol/L磷酸二氢钾溶液－甲醇（75：25），流速1.0 mL/min，检测波长210 nm。结果盐酸麻黄碱质量浓度在1.06~21.1μg/mL范围内与峰面积呈良好的线性关系( r2=0.9999)，最低检测限为1.06 ng/mL。平均回收率分别为100.54%，99.86%，100.20%；RSD为0.90%，0.37%，0.43%( n=9)。结论该方法专属性好，准确、灵敏，用于测定复方甘草麻黄碱片中麻黄碱的含量,结果可靠。
목적：건립측정복방감초마황감편중마황감함량적방법。방법채용반상고효액상색보법，색보주위 Diamonsil (찬석) C18주(250 mm ×4.6 mm，5μm），류동상위0.1 mol/L린산이경갑용액－갑순（75：25），류속1.0 mL/min，검측파장210 nm。결과염산마황감질량농도재1.06~21.1μg/mL범위내여봉면적정량호적선성관계( r2=0.9999)，최저검측한위1.06 ng/mL。평균회수솔분별위100.54%，99.86%，100.20%；RSD위0.90%，0.37%，0.43%( n=9)。결론해방법전속성호，준학、령민，용우측정복방감초마황감편중마황감적함량,결과가고。
Objective To establish a RP-HPLC method for determining the ephedrine hydrochloride content in Compound Glycyrrhiza Ephedrine Tablets. Methods The RP-HPLC method was adopted with the Diamonsil C18 Column ( 250 mm × 4. 6 mm, 5 μm ) and the 0. 1 mol/L potassium dihydrogen phosphate solution-methanol ( 75:25 ) as the mobile phase at the flow rate of 1. 0 mL/min. The detec-tion wavelength was 210 nm. Results The linear range of ephedrine hydrochloride was 1. 06-21. 1μg/mL ( r2=0. 999 9 ) , with the low-est limit of the detection 1. 06 ng/mL; the average recovery rate of high, middle and low concentrations were 100. 54% , 99. 86% , 100. 20% ( RSD=0. 90%, 0. 37%, 0. 43% ) respectively. Conclusion This method is more specific, accurate, sensitive and reliable for the determination of the ephedrine hydrochloride content in Compound Glycyrrhiza Ephedrine Tablets.