CAJ | 학술논문

应用超高效液相色谱－串联质谱技术建立了兽用中药制剂穿心莲注射液和鱼腥草注射液中非法添加庆大霉素的检测方法。样品经水溶解后稀释，采用20 mmol／L七氟丁酸（ HFBA）乙腈与20 mmol／L HFBA水溶液作为流动相进行梯度洗脱，经ACQUITY HSS PFP色谱柱分离，通过多反应监测模式进行测定。庆大霉素在0．02～2μg／mL的范围内线性关系良好，相关系数0．9998。平均回收率为98．2％～100．3％，相对标准偏差小于2．4％。检测限为2 mg／mL，定量限为4 mg／mL，该法准确、简单、快速。
응용초고효액상색보－천련질보기술건립료수용중약제제천심련주사액화어성초주사액중비법첨가경대매소적검측방법。양품경수용해후희석，채용20 mmol／L칠불정산（ HFBA）을정여20 mmol／L HFBA수용액작위류동상진행제도세탈，경ACQUITY HSS PFP색보주분리，통과다반응감측모식진행측정。경대매소재0．02～2μg／mL적범위내선성관계량호，상관계수0．9998。평균회수솔위98．2％～100．3％，상대표준편차소우2．4％。검측한위2 mg／mL，정량한위4 mg／mL，해법준학、간단、쾌속。
A method of Ultra-HPLC-MS/MS was developed to determine gentamycin illegally added in traditional Chinese medicine for animals. The samples were dissolved and diluted. The HPLC separation was performed on ACQUITY HSS PFP column with a linear gradient elution program of 20 mmol/L HFBA acetonitrile and 20 mmol/L HFBA aqueous solution as the mobile phase. Selective reaction monitoring was used for the selective detection. The linearity of gentamycin ranged from 0.02 to 2μg/mL with coefficient 0.9998. The average recovery was within the range of 98.2%~100.3% and RSD were less than 2.4%. The limit of detection was 2 mg/mL and limit of quantification was 4 mg/mL. The method was accurate,convenient and fast.