中国兽药杂志
中國獸藥雜誌
중국수약잡지
CHINESE JOURNAL OF VETERINARY DRUG
2015年
1期
19-23
,共5页
红霉素%常压室温等离子体%紫外%复合诱变%遗传稳定性
紅黴素%常壓室溫等離子體%紫外%複閤誘變%遺傳穩定性
홍매소%상압실온등리자체%자외%복합유변%유전은정성
erythromycin%atmospheric and room temperature plasma(ARTP)%ultraviolet(uv)%composite mutagenesis%hereditary stability
利用常压室温等离子体射流诱变（ ARTP ）和紫外照射对红霉素产生菌进行复合诱变，得到4株产量明显提高的突变菌株，4株菌的平均发酵效价较出发菌株提高25．2％；其中一株（12＃菌株）经发酵摇瓶验证，红霉素发酵单位可达10029单位／mL，比出发菌株提高了28．6％，且遗传稳定性良好。实验证明ARTP－UV复合诱变是一种简单高效的筛选方法。
利用常壓室溫等離子體射流誘變（ ARTP ）和紫外照射對紅黴素產生菌進行複閤誘變，得到4株產量明顯提高的突變菌株，4株菌的平均髮酵效價較齣髮菌株提高25．2％；其中一株（12＃菌株）經髮酵搖瓶驗證，紅黴素髮酵單位可達10029單位／mL，比齣髮菌株提高瞭28．6％，且遺傳穩定性良好。實驗證明ARTP－UV複閤誘變是一種簡單高效的篩選方法。
이용상압실온등리자체사류유변（ ARTP ）화자외조사대홍매소산생균진행복합유변，득도4주산량명현제고적돌변균주，4주균적평균발효효개교출발균주제고25．2％；기중일주（12＃균주）경발효요병험증，홍매소발효단위가체10029단위／mL，비출발균주제고료28．6％，차유전은정성량호。실험증명ARTP－UV복합유변시일충간단고효적사선방법。
Atmospheric and room temperature plasma( ARTP )-UV composite mutagenesis were adopted to treat with erythromycin-producing strain,four high-efficient producing strains were obtained by screening,which was increased in fermentation potency by 25. 2% over the original strain. The mutant 12# with genetic stability was obtained. Its yield of erythromycin was 10029 U/mL, 28.6% higher than the original strain. ARTP-UV composite mutation was a simple and efficient method for screening.
