中国兽药杂志
中國獸藥雜誌
중국수약잡지
CHINESE JOURNAL OF VETERINARY DRUG
2015年
1期
10-13
,共4页
李建%廖园园%秦红刚%刘洁%朱薇%漆世华%韩兴%徐松%舒银辉%谢红玲
李建%廖園園%秦紅剛%劉潔%硃薇%漆世華%韓興%徐鬆%舒銀輝%謝紅玲
리건%료완완%진홍강%류길%주미%칠세화%한흥%서송%서은휘%사홍령
日本乙型脑炎病毒%间接ELISA%试剂盒%抗体
日本乙型腦炎病毒%間接ELISA%試劑盒%抗體
일본을형뇌염병독%간접ELISA%시제합%항체
Japanese encephalitis virus%indirect ELISA%kits%antibody
采用日本乙型脑炎病毒单克隆抗体预包被酶标板，将纯化的日本乙型脑炎病毒（ JEV）作为检测用抗原，利用包被捕获法建立了用于检测猪乙型脑炎抗体的间接ELISA法。采用建立的ELISA法对50份已知阴性血清样本检测，临界OD450nm值为0．343，ELISA与IFA对200份血清进行平行检测，总符合率为92．9％，与商品化的同类国产试剂盒的符合率为95％。与其他常见的猪病毒阳性血清抗体无交叉反应，2～8℃保存12个月稳定。研制的ELISA抗体检测试剂盒为临床JEV血清抗体检测及其疫苗的免疫效果评价提供了技术手段。
採用日本乙型腦炎病毒單剋隆抗體預包被酶標闆，將純化的日本乙型腦炎病毒（ JEV）作為檢測用抗原，利用包被捕穫法建立瞭用于檢測豬乙型腦炎抗體的間接ELISA法。採用建立的ELISA法對50份已知陰性血清樣本檢測，臨界OD450nm值為0．343，ELISA與IFA對200份血清進行平行檢測，總符閤率為92．9％，與商品化的同類國產試劑盒的符閤率為95％。與其他常見的豬病毒暘性血清抗體無交扠反應，2～8℃保存12箇月穩定。研製的ELISA抗體檢測試劑盒為臨床JEV血清抗體檢測及其疫苗的免疫效果評價提供瞭技術手段。
채용일본을형뇌염병독단극륭항체예포피매표판，장순화적일본을형뇌염병독（ JEV）작위검측용항원，이용포피포획법건립료용우검측저을형뇌염항체적간접ELISA법。채용건립적ELISA법대50빈이지음성혈청양본검측，림계OD450nm치위0．343，ELISA여IFA대200빈혈청진행평행검측，총부합솔위92．9％，여상품화적동류국산시제합적부합솔위95％。여기타상견적저병독양성혈청항체무교차반응，2～8℃보존12개월은정。연제적ELISA항체검측시제합위림상JEV혈청항체검측급기역묘적면역효과평개제공료기술수단。
With capture coating method, indirect ELISA method for detecting Japanese encephlitis antibody was established using Japanese encephlitis virus monoclonal antibody to pre-coat ELISA plate and using purified Japanese encephlitis as detecting antigen. The ELISA was optimized and the kit was developed for checking the immune results of JEV vaccines. In comparison with inderect immunofluorescence ( IFA ) , there was 92. 9%coincidence between the ELISA and IFA based on detection of 200 serum samples. With the commercialization of the same kind of domestic kits,there was 95% coincidence.There was no cross reactions for the positive serums of other common Japanese encephlitis antibodies.The kit was stable kept up to 12 months at 2~8℃.The kit was used for clinical laboratory and detection of serum antibody.
