中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
24期
35-37
,共3页
壮尔颗粒%流式细胞术%免疫器官指数%T淋巴细胞亚群
壯爾顆粒%流式細胞術%免疫器官指數%T淋巴細胞亞群
장이과립%류식세포술%면역기관지수%T림파세포아군
Zhuang'er Granules%flow cytometry%immune organ index%T-lymphocyte subsets
目的：观察壮尔颗粒（Zhuang'er Granules，ZG）对细胞免疫抑制小鼠免疫器官指数及T淋巴细胞亚群的影响。方法用环孢菌素A （CsA）建立小鼠模型，并在检测前第7天滴鼻肺炎链球菌（SP）。灌胃给药17 d后，测定小鼠免疫器官指数，并采用流式细胞仪（FACS）检测T淋巴细胞亚群的变化。结果胸腺指数与脾脏指数同空白对照组相比，模型组均有明显下降（ P﹤0.05），各给药组与模型组比较均有明显提高（ P﹤0.05），并随着给药量的增加而升高（ P﹤0.05）。脾脏中CD3+，CD4+，CD8+与空白对照组相比，模型组CD3+明显下降， CD4+有下降趋势，CD8+有上升趋势；各给药组CD3+，CD4+百分率随着给药剂量增加而升高，而CD8+的百分率随着给药剂量增加而降低。血中CD3+，CD4+，CD8+与空白对照组相比，模型组CD3+有下降趋势，CD4+明显下降（ P﹤0.05），CD8+明显升高（ P﹤0.05），各给药组CD3+和CD4+的百分率随着给药剂量的增加而升高（ P﹤0.05），而CD8+的百分率随着给药剂量的增加而降低。结论壮尔颗粒有提高小鼠免疫功能的作用，提高CD3+和CD4+的同时，降低CD8+，以通过T细胞的平衡来调节小鼠自身的免疫功能。
目的：觀察壯爾顆粒（Zhuang'er Granules，ZG）對細胞免疫抑製小鼠免疫器官指數及T淋巴細胞亞群的影響。方法用環孢菌素A （CsA）建立小鼠模型，併在檢測前第7天滴鼻肺炎鏈毬菌（SP）。灌胃給藥17 d後，測定小鼠免疫器官指數，併採用流式細胞儀（FACS）檢測T淋巴細胞亞群的變化。結果胸腺指數與脾髒指數同空白對照組相比，模型組均有明顯下降（ P﹤0.05），各給藥組與模型組比較均有明顯提高（ P﹤0.05），併隨著給藥量的增加而升高（ P﹤0.05）。脾髒中CD3+，CD4+，CD8+與空白對照組相比，模型組CD3+明顯下降， CD4+有下降趨勢，CD8+有上升趨勢；各給藥組CD3+，CD4+百分率隨著給藥劑量增加而升高，而CD8+的百分率隨著給藥劑量增加而降低。血中CD3+，CD4+，CD8+與空白對照組相比，模型組CD3+有下降趨勢，CD4+明顯下降（ P﹤0.05），CD8+明顯升高（ P﹤0.05），各給藥組CD3+和CD4+的百分率隨著給藥劑量的增加而升高（ P﹤0.05），而CD8+的百分率隨著給藥劑量的增加而降低。結論壯爾顆粒有提高小鼠免疫功能的作用，提高CD3+和CD4+的同時，降低CD8+，以通過T細胞的平衡來調節小鼠自身的免疫功能。
목적：관찰장이과립（Zhuang'er Granules，ZG）대세포면역억제소서면역기관지수급T림파세포아군적영향。방법용배포균소A （CsA）건립소서모형，병재검측전제7천적비폐염련구균（SP）。관위급약17 d후，측정소서면역기관지수，병채용류식세포의（FACS）검측T림파세포아군적변화。결과흉선지수여비장지수동공백대조조상비，모형조균유명현하강（ P﹤0.05），각급약조여모형조비교균유명현제고（ P﹤0.05），병수착급약량적증가이승고（ P﹤0.05）。비장중CD3+，CD4+，CD8+여공백대조조상비，모형조CD3+명현하강， CD4+유하강추세，CD8+유상승추세；각급약조CD3+，CD4+백분솔수착급약제량증가이승고，이CD8+적백분솔수착급약제량증가이강저。혈중CD3+，CD4+，CD8+여공백대조조상비，모형조CD3+유하강추세，CD4+명현하강（ P﹤0.05），CD8+명현승고（ P﹤0.05），각급약조CD3+화CD4+적백분솔수착급약제량적증가이승고（ P﹤0.05），이CD8+적백분솔수착급약제량적증가이강저。결론장이과립유제고소서면역공능적작용，제고CD3+화CD4+적동시，강저CD8+，이통과T세포적평형래조절소서자신적면역공능。
Objective To observe the effect of Zhuang'er Granules on the immune organ index and T-lymphocytes subsets in the cellu-lar immunosupressed mice. Methods The mouse model was established by cyclosporin A(CsA) and given Streptococcus pneumoniae (SP) by nasal drops on 7 d before detection. The mouse immune organ index was determined after 17 d intragastric gavage and the changes of T-lymphocyte subsets were detected by the flow cytometry(FACS). Results Compared with the blank control group,the thymus index and spleen index in the model group were obviously decreased( P ﹤ 0. 05);compared with the model group,which in each dose group were obviously increased( P ﹤ 0. 05),and obviously increased with dose increase( P ﹤ 0. 05);compared with the blank control group,for CD3 +,CD4 +,CD8+ in the spleen,CD3 + in the model group was declined obviously( P ﹤ 0. 05),CD4 + showed the de-cline trend and CD8 + model group had the rising trend;the percentage of CD3 +,CD4 + in each dose group was increased with dose in-crease,while the percentage of CD8+ was declined with dose increase;compared with the blank control group,for blood CD3 +,CD4 +, CD8 +,CD3+ in the model group showed the decline trend,CD4+ was obviously decreased( P ﹤ 0. 05)and CD8 + was obviously increased ( P ﹤ 0. 05),the percentage of CD3 +,CD4 + in each medication group was increased with dose increase( P ﹤ 0. 05),while the percent-age of CD8 + in each medication group was decreased with dose increase. Conclusion Zhuang'er Granules has the effect for improving the mice immune function,increases CD3 + and CD4+,simultaneously decreases CD8+,which could regulate the mice immune function via T-lymphocytes balance.
