CAJ | 학술논문

研究了5-羟色胺(5-HT)对马氏珠母贝解剖获得卵母细胞的促熟作用及5-HT受体(5-HTpf)在其性腺中的分布情况。通过体外浸泡的方法处理马氏珠母贝卵母细胞使其成熟，结果表明一定浓度的5-HT可显著促使马氏珠母贝卵母细胞生发泡破裂，并使已经发生生发泡破裂的卵母细胞进一步成熟，同时可诱导5-HT受体基因的表达。用原位杂交的方法对5-HT受体在马氏珠母贝性腺中的分布的研究结果揭示，卵巢中次级卵母细胞和卵细胞均存在5-HT受体，有阳性信号出现；精巢中仅滤泡壁及精母细胞中有少量表达。以上结果表明马氏珠母贝的卵母细胞不仅能产生5-HT并且可表达5-HT受体。
연구료5-간색알(5-HT)대마씨주모패해부획득란모세포적촉숙작용급5-HT수체(5-HTpf)재기성선중적분포정황。통과체외침포적방법처리마씨주모패란모세포사기성숙，결과표명일정농도적5-HT가현저촉사마씨주모패란모세포생발포파렬，병사이경발생생발포파렬적란모세포진일보성숙，동시가유도5-HT수체기인적표체。용원위잡교적방법대5-HT수체재마씨주모패성선중적분포적연구결과게시，란소중차급란모세포화란세포균존재5-HT수체，유양성신호출현；정소중부려포벽급정모세포중유소량표체。이상결과표명마씨주모패적란모세포불부능산생5-HT병차가표체5-HT수체。
To investigate the effect of 5-HT on the maturation of oocyte stripped from the pearl oyster Pinctada fucata, oocytes were treated with 5-HT solution in vitro. Results showed that 5-HT was able to induce not only the Germinal Vesicle Breakdown (GVBD) of the oocytes remarkably, but also the maturation of the oocytes already GVBD. Gene expression of the 5-HT receptor was able to be induced at the same time. Distribution of 5-HT receptor (5-HTpf) within gonads of P. fucata was also studied using in situ hybridization (ISH) technique. The results revealed that 5-HT receptor 5-HTpf existed in the oocytes of different development stages and in the spermatocytes, and the reactive substances of 5-HTpf distributed along the cytoplasm and membrane of oocytes and showed blue. The above results indicate that the oocytes of P.fucata can not only produce 5-HT, but also express its receptor.