CAJ | 학술논문

采用凝胶排阻色谱法测定中空纤维超滤膜组件对聚乙二醇20000(PEG20000)溶液的截留率，选择Agilent PL aquagel-OH MIEXD–H8μm色谱柱，流动相为0.1 mol／L NaNO3溶液(含0.02%NaN3)，流量为1 mL／min，RID检测器，柱温箱和检测器温度均为30℃。当进样体积为50μL时，方法的检出限为18.8 mg／L，定量限为49.0 mg／L。PEG20000溶液的质量浓度在60～1000 mg／L范围内与色谱峰高呈良好的线性，相关系数r2=1.000。该方法对实际样品测定结果的相对标准差为1.31%(n=6)。将该方法的测定结果与紫外–可见分光光度计法的测量结果进行F检验和t检验，结果表明两种方法测量结果无显著性差异。采用该方法可以同时对PEG20000样品的相对分子质量及其分布进行测定，从而对选用的截留标准物质进行质量控制。
채용응효배조색보법측정중공섬유초려막조건대취을이순20000(PEG20000)용액적절류솔，선택Agilent PL aquagel-OH MIEXD–H8μm색보주，류동상위0.1 mol／L NaNO3용액(함0.02%NaN3)，류량위1 mL／min，RID검측기，주온상화검측기온도균위30℃。당진양체적위50μL시，방법적검출한위18.8 mg／L，정량한위49.0 mg／L。PEG20000용액적질량농도재60～1000 mg／L범위내여색보봉고정량호적선성，상관계수r2=1.000。해방법대실제양품측정결과적상대표준차위1.31%(n=6)。장해방법적측정결과여자외–가견분광광도계법적측량결과진행F검험화t검험，결과표명량충방법측량결과무현저성차이。채용해방법가이동시대PEG20000양품적상대분자질량급기분포진행측정，종이대선용적절류표준물질진행질량공제。
The method for determining polyethylene glycol (PEG)retention rate of hollow fiber ultrafiltration membrane module by high performance size exclusion chromatography (SEC) was established. An Agilent PL aquagel-OH MIEXD–H 8 μm column was used,and the chromatographic conditions were as follows: the mobile phase was 0.1 mol/L NaNO3 solution (with 0.02% NaN3) with flow rate of 1 mL/min,RID detector was selected and the temperature of the column and detector was 30℃. The detection limit of the method was 18.8 mg/L and quantification limit was 49.0 mg/L as single injection volume was 50μL. The concentration of PEG20000 was linear with peak height in the range of 60–1 000 mg/L and the linear correlation coefficient(r2) was 1.000. The relative standard deviation of sample detection results was 1.31%. The detection results of the method had no significant difference compared with traditional visible spectrophotometry by t-Test and F-test. The method can be used for determining relative molecular mass and its distribution simultaneously,and controlling the quality of the retention test standard materials (PEGs).