器官移植
器官移植
기관이식
OGRAN TRANSPLANTATION
2015年
1期
26-30
,共5页
谢江平%张夕凉%刘刚%吴仕和%王育红
謝江平%張夕涼%劉剛%吳仕和%王育紅
사강평%장석량%류강%오사화%왕육홍
调节性T细胞%小鼠%心脏移植%西罗莫司%环孢素
調節性T細胞%小鼠%心髒移植%西囉莫司%環孢素
조절성T세포%소서%심장이식%서라막사%배포소
Regulatory T cell%Mouse%Heart transplantation%Sirolimus%Ciclosporine
目的：探讨西罗莫司（SRL）对小鼠异位心脏移植模型的移植物存活时间以及脾脏中调节性T细胞（Treg）分化和增殖的影响。方法应用Cuff法建立雄性BALB／c→C57BL／6小鼠颈部异位心脏移植模型。术后随机分为3组各10只受体：对照组术后不予特殊药物治疗，SRL组术后1～14 d予SRL 10 mg／（kg·d）灌胃，环孢素（CsA）组术后1～14 d 予CsA 30 mg／（kg·d）灌胃。记录移植心脏存活时间，于移植心停搏或术后14 d取脾，分离单个核细胞，上流式细胞仪检测CD4＋CD25＋Treg占CD4＋T细胞比例（CD4＋CD25＋Treg％），采用逆转录-聚合酶链反应（RT-PCR）法半定量检测Foxp3信使核糖核酸（mRNA）表达水平。结果与对照组比较，CsA组和SRL组均明显延长小鼠移植心的生存时间（均为P＜0.01），而两者之间比较差异无统计学意义（P＞0.05）。与对照组比较，CsA组脾脏中CD4＋CD25＋Treg％明显降低，而SRL组则明显升高（均为P＜0.01），后两组比较差异有统计学意义（P＜0.01）。SRL组脾脏T细胞的Foxp3 mRNA表达明显高于对照组和CsA组，其中对照组亦明显高于CsA组（均为P＜0.01）。结论在小鼠心脏移植模型中，SRL明显延长移植物的存活期，促进CD4＋CD25＋Treg的增殖与生长，有利于免疫耐受的形成。
目的：探討西囉莫司（SRL）對小鼠異位心髒移植模型的移植物存活時間以及脾髒中調節性T細胞（Treg）分化和增殖的影響。方法應用Cuff法建立雄性BALB／c→C57BL／6小鼠頸部異位心髒移植模型。術後隨機分為3組各10隻受體：對照組術後不予特殊藥物治療，SRL組術後1～14 d予SRL 10 mg／（kg·d）灌胃，環孢素（CsA）組術後1～14 d 予CsA 30 mg／（kg·d）灌胃。記錄移植心髒存活時間，于移植心停搏或術後14 d取脾，分離單箇覈細胞，上流式細胞儀檢測CD4＋CD25＋Treg佔CD4＋T細胞比例（CD4＋CD25＋Treg％），採用逆轉錄-聚閤酶鏈反應（RT-PCR）法半定量檢測Foxp3信使覈糖覈痠（mRNA）錶達水平。結果與對照組比較，CsA組和SRL組均明顯延長小鼠移植心的生存時間（均為P＜0.01），而兩者之間比較差異無統計學意義（P＞0.05）。與對照組比較，CsA組脾髒中CD4＋CD25＋Treg％明顯降低，而SRL組則明顯升高（均為P＜0.01），後兩組比較差異有統計學意義（P＜0.01）。SRL組脾髒T細胞的Foxp3 mRNA錶達明顯高于對照組和CsA組，其中對照組亦明顯高于CsA組（均為P＜0.01）。結論在小鼠心髒移植模型中，SRL明顯延長移植物的存活期，促進CD4＋CD25＋Treg的增殖與生長，有利于免疫耐受的形成。
목적：탐토서라막사（SRL）대소서이위심장이식모형적이식물존활시간이급비장중조절성T세포（Treg）분화화증식적영향。방법응용Cuff법건립웅성BALB／c→C57BL／6소서경부이위심장이식모형。술후수궤분위3조각10지수체：대조조술후불여특수약물치료，SRL조술후1～14 d여SRL 10 mg／（kg·d）관위，배포소（CsA）조술후1～14 d 여CsA 30 mg／（kg·d）관위。기록이식심장존활시간，우이식심정박혹술후14 d취비，분리단개핵세포，상류식세포의검측CD4＋CD25＋Treg점CD4＋T세포비례（CD4＋CD25＋Treg％），채용역전록-취합매련반응（RT-PCR）법반정량검측Foxp3신사핵당핵산（mRNA）표체수평。결과여대조조비교，CsA조화SRL조균명현연장소서이식심적생존시간（균위P＜0.01），이량자지간비교차이무통계학의의（P＞0.05）。여대조조비교，CsA조비장중CD4＋CD25＋Treg％명현강저，이SRL조칙명현승고（균위P＜0.01），후량조비교차이유통계학의의（P＜0.01）。SRL조비장T세포적Foxp3 mRNA표체명현고우대조조화CsA조，기중대조조역명현고우CsA조（균위P＜0.01）。결론재소서심장이식모형중，SRL명현연장이식물적존활기，촉진CD4＋CD25＋Treg적증식여생장，유리우면역내수적형성。
Objective To investigate the impacts of sirolimus (SRL)on the survival time of graft and the differentiation and proliferation of regulatory T cell (Treg ) of spleen in mouse heterotopic heart transplantation model. Methods Male BALB /c → C57BL/6 mice cervical heterotopic heart transplantation model was established by Cuff method. The mice were divided into 3 groups randomly with 10 mice in each group. The control group received no treatment of special medicine after operation. Mice in SRL group were gavaged with SRL 10 mg/(kg·d)at 1-14 d after operation. Mice in ciclosporin (CsA)group were gavaged with CsA 30 mg/(kg·d) at 1-14 d after operation. The survival time of cardiac grafts were recorded. The spleen was procured after asystole of cardiac graft or 14 d after operation. Mononuclear cells were isolated and the proportion of CD4 +CD25 +Treg in CD4 +T cell (CD4 +CD25 +Treg%)were detected by flow cytometry and reverse transcription polymerase chain reaction (RT-PCR)was used to examine the expression of Foxp3 messenger ribonucleic acid (mRNA ) semi-quantitatively. Results Compared with the control group,the survival time of cardiac grafts prolonged significantly in SRL and CsA group (all in P <0.01 ),but no significant difference was observed between SRL and CsA group (P>0.05 ). Compared with the control group,CD4 +CD25 +Treg% significantly decreased in the spleen of CsA group and significantly increased in SRL group (all in P<0.01 ). And significant difference was observed between SRL and CsA group (P<0.01). Expression of Foxp3 mRNA of T lymphocyte in the spleen of SRL group was significantly higher than those in control and CsA group (P<0.01). And expression of Foxp3 mRNA in control group was significantly higher than that in CsA group (P<0.01 ). Conclusions In mouse heart transplantation model,SRL can prolong the survival time of graft and promote the proliferation and growth of CD4 +CD25 +Treg to facilitate the establishment of immune tolerance.
