CAJ | 학술논문

在吉林省4个生态类型地区采集了74株不同建植年限苜蓿的根瘤,调查了“公农1号”紫花苜蓿根瘤菌株的分布情况。结果表明,苜蓿根瘤多数分布在根部10 cm 以下的须根；根瘤数量、重量受苜蓿建植年限、不同生态类型及土壤类型的影响较大。分离纯化所得的81株根瘤分离物经“公农1号”苜蓿水培回接结瘤试验,得到 bc8,gzl59和 da80等8个优良根瘤菌株；在温室条件下,对回接试验筛选出的8 个菌株进行土壤盆栽复筛试验,以期筛选出适合“公农1号”苜蓿生长的高效根瘤菌株。结果表明,根瘤分布在植株根部10~30 cm 处；在所有供试菌株中,菌株 bc18、yj34、bc94和 gzl59表现较为优良。菌株 yj34和 bc94对茎长和叶片数影响最大,茎长分别增加60.17%和59.86%,叶片数分别增加97.92%和95.92%；菌株 gzl59对根长影响最大,根长增加64.32%；菌株 gzl59和 bc94对提高植株干重贡献最大,其中,菌株 gzl59比对照增产288.24%；供试菌株对根瘤数的影响也很显著,接种菌株 yj34的苜蓿根瘤数相比其他菌株较高,达104.24%。综合分析,发现根瘤菌株 gzl59、bc94和 yj34较其他菌株表现突出,是吉林省“公农1号”苜蓿的高效根瘤菌株。
재길림성4개생태류형지구채집료74주불동건식년한목숙적근류,조사료“공농1호”자화목숙근류균주적분포정황。결과표명,목숙근류다수분포재근부10 cm 이하적수근；근류수량、중량수목숙건식년한、불동생태류형급토양류형적영향교대。분리순화소득적81주근류분리물경“공농1호”목숙수배회접결류시험,득도 bc8,gzl59화 da80등8개우량근류균주；재온실조건하,대회접시험사선출적8 개균주진행토양분재복사시험,이기사선출괄합“공농1호”목숙생장적고효근류균주。결과표명,근류분포재식주근부10~30 cm 처；재소유공시균주중,균주 bc18、yj34、bc94화 gzl59표현교위우량。균주 yj34화 bc94대경장화협편수영향최대,경장분별증가60.17%화59.86%,협편수분별증가97.92%화95.92%；균주 gzl59대근장영향최대,근장증가64.32%；균주 gzl59화 bc94대제고식주간중공헌최대,기중,균주 gzl59비대조증산288.24%；공시균주대근류수적영향야흔현저,접충균주 yj34적목숙근류수상비기타균주교고,체104.24%。종합분석,발현근류균주 gzl59、bc94화 yj34교기타균주표현돌출,시길림성“공농1호”목숙적고효근류균주。
We collected root nodules of 74 alfalfa plants in four different ecological regions in Jilin Province to survey rhizobia resources of ‘Gongnong No.1’alfalfa.The results showed that root nodules were generally located in 10 cm below the fibrous roots.The nodule number and the nodule weight in various places were influ-enced by the implantation time,the ecological type,and the soil type.81 fast-growing isolates from the root nod-ules were obtained.Eight rhizobial strains such as bc8,bc18,gzl59,da80,etc.were screened out by the tieback nodulation water culture,which were used to screen high efficient strains for ‘Gongnong No.1 ’alfalfa in the greenhouse study.The results showed that the after 75 days of greenhouse cultivation,most of nodules distribu-ted in 10 to 30 cm of the roots;the stem length,root length,number of leaves,dry weight and nodule number per plant of alfalfa inoculated with rhizobia were all increased significantly(P <0.05).Compared to the control,the stem length increased by 60.17% and 59.86% ,and the number of leaves increased 97.92% and 95.92% by in-oculated with yj34 and bc94,respectively;The root length was raised up 64.32% by gzl59;The dry weight of the plant was 288.24% and 157.14% higher than the control inoculated with gzl59 and bc54,respectively;Besides, the inoculated strains increased the nodule number per plant as well,the alfalfa plant formed more nodules by inoculated with strain yj34 than other test strains.Among 8 strains selected,strains bc18,yj34,bc94 and gzl59 on alfalfa were more effective than others;Of the four strains,the performance of three indigenous strains bc94 and gzl59 from its host of alfalfa grew in sandy loam soil in Baicheng and Gong Zhuling,yj34 from its host of al-falfa grew in dark brown soil in a ravine of Yanji exceeded the other strains estimated by plant growth.They are the highly-effictive rhizobial strains on ‘Gongnong No.1’alfalfa in Jilin Province.