草原与草坪
草原與草坪
초원여초평
GRASSLAND AND TURF
2014年
6期
10-17
,共8页
苜蓿根瘤菌%稀土盐类%植物源抑菌剂%筛选%耐药性
苜蓿根瘤菌%稀土鹽類%植物源抑菌劑%篩選%耐藥性
목숙근류균%희토염류%식물원억균제%사선%내약성
Rhizobia meliloti%rare earth salts%botanical antimicrobial%physiological and biochemical charac-teristics test%antimicrobial resistance
为明确荧光蛋白标记技术对根瘤菌耐药性的影响,采集6个品种的苜蓿根瘤进行根瘤菌的分离、纯化和初步鉴定,并筛选其中对3种稀土盐抑菌剂 La(NO3)3·6 H 2 O,LaCl3和 Ce(NO3)3·6 H 2 O以及2种植物源抑菌剂苦参碱和除虫菊素具有耐受性的菌株,得到一株编号为 LH3436的根瘤菌,对0.6 mg/mL 苦参碱具有天然耐受性。将 LH3436和对照根瘤菌 R.12531以及参比根瘤菌 R.GN5进行荧光蛋白标记后,观察其对抑菌剂耐受性的变化。发现各初筛菌株在无氮培养基上的生长状况对非根瘤菌的筛除率最高,所有初筛菌株均为革兰氏染色阴性的快生型产酸菌,全部难以利用柠檬酸盐,而可以利用蔗糖和可溶性淀粉,但对乳糖、葡萄糖和果糖等碳源的利用存在差异性；试验初筛菌株对植物源类抑菌剂的相对耐受性好,而对稀土盐类抑菌剂的相对耐受性差,将筛选根瘤菌进行荧光蛋白标记后,其对抑菌剂的耐受性未发生改变。
為明確熒光蛋白標記技術對根瘤菌耐藥性的影響,採集6箇品種的苜蓿根瘤進行根瘤菌的分離、純化和初步鑒定,併篩選其中對3種稀土鹽抑菌劑 La(NO3)3·6 H 2 O,LaCl3和 Ce(NO3)3·6 H 2 O以及2種植物源抑菌劑苦參堿和除蟲菊素具有耐受性的菌株,得到一株編號為 LH3436的根瘤菌,對0.6 mg/mL 苦參堿具有天然耐受性。將 LH3436和對照根瘤菌 R.12531以及參比根瘤菌 R.GN5進行熒光蛋白標記後,觀察其對抑菌劑耐受性的變化。髮現各初篩菌株在無氮培養基上的生長狀況對非根瘤菌的篩除率最高,所有初篩菌株均為革蘭氏染色陰性的快生型產痠菌,全部難以利用檸檬痠鹽,而可以利用蔗糖和可溶性澱粉,但對乳糖、葡萄糖和果糖等碳源的利用存在差異性；試驗初篩菌株對植物源類抑菌劑的相對耐受性好,而對稀土鹽類抑菌劑的相對耐受性差,將篩選根瘤菌進行熒光蛋白標記後,其對抑菌劑的耐受性未髮生改變。
위명학형광단백표기기술대근류균내약성적영향,채집6개품충적목숙근류진행근류균적분리、순화화초보감정,병사선기중대3충희토염억균제 La(NO3)3·6 H 2 O,LaCl3화 Ce(NO3)3·6 H 2 O이급2충식물원억균제고삼감화제충국소구유내수성적균주,득도일주편호위 LH3436적근류균,대0.6 mg/mL 고삼감구유천연내수성。장 LH3436화대조근류균 R.12531이급삼비근류균 R.GN5진행형광단백표기후,관찰기대억균제내수성적변화。발현각초사균주재무담배양기상적생장상황대비근류균적사제솔최고,소유초사균주균위혁란씨염색음성적쾌생형산산균,전부난이이용저몽산염,이가이이용자당화가용성정분,단대유당、포도당화과당등탄원적이용존재차이성；시험초사균주대식물원류억균제적상대내수성호,이대희토염류억균제적상대내수성차,장사선근류균진행형광단백표기후,기대억균제적내수성미발생개변。
In order to clarify the effect of fluorescent protein marker technology on rhizobia resistance to an-timicrobials,the nodules of 6 alfalfa varieties were collected for rhizobia isolation and identification.The prelimi-narily isolated rhizobia were tested in terms of their resistance to 3 rare earth salts (La(NO3 )3 ·6 H 2 O,LaCl3 and Ce (NO3 )3 · 6 H 2 O)and 2 botanical antimicrobials (matrine and pyrethrin).The obtained rhizobium LH3436 was resistant to 0.6 mg/mL matrine.LH3436 and the control rhizobia R.12531 and R.GN5 were marked with fluorescence protein and their resistance variance to antimicrobials was tested.The results indicated that the growth status of the preliminarily screened rhizobia on nitrogen free medium showed the highest screen-ing rate,all the preliminarily screened rhizobia were gram negative and fast growing acid forming bacteria,all were incapable of using citrate,but could use sucrose and soluble starch,differences existed among the rhizobia in the utilization of lactose,glucose and fructose.All the screened rhizobia showed relatively better tolerance to botanical antimicrobials,but poorer tolerance to rare earth salts.Fluorescence protein marker technology did not show influence on rhizobia resistance to antimicrobials.
