CAJ | 학술논문

在高密度工业化封闭循环海水养殖条件下（养殖密度为14?2±0?48 kg／m2），选取大菱鲆（371?68±43?15 g）360尾，随机均分4组，设置4个水温梯度14℃、16℃、18℃和21℃，旨在探寻水温对大菱鲆氨氮排泄、转氨酶及血清免疫因子的影响，试验期为56 d。结果表明：（1）养殖水体总氨氮浓度随水温升高呈先升后降，18℃时总氨氮浓度极显著高于14℃、21℃时；大菱鲆氨氮排泄率随水温升高而升高，且二者呈指数关系。（2）大菱鲆肝脏GPT、GOT活力随水温升高呈先升后降，均在18℃达最大值；而血清两种指标变化趋势与之相反，均在21℃达最大值。（3）血清LZM、SOD活力及皮质醇含量均随水温升高呈先升后降，均在18℃达最大值；血氨含量则逐渐下降，14℃时极显著高于18℃、21℃时；鳃丝Na＋?K＋?ATPase活力随水温升高而升高，但组间差异不显著。综上，封闭循环水养殖大菱鲆的适宜水温为16～18℃。
재고밀도공업화봉폐순배해수양식조건하（양식밀도위14?2±0?48 kg／m2），선취대릉평（371?68±43?15 g）360미，수궤균분4조，설치4개수온제도14℃、16℃、18℃화21℃，지재탐심수온대대릉평안담배설、전안매급혈청면역인자적영향，시험기위56 d。결과표명：（1）양식수체총안담농도수수온승고정선승후강，18℃시총안담농도겁현저고우14℃、21℃시；대릉평안담배설솔수수온승고이승고，차이자정지수관계。（2）대릉평간장GPT、GOT활력수수온승고정선승후강，균재18℃체최대치；이혈청량충지표변화추세여지상반，균재21℃체최대치。（3）혈청LZM、SOD활력급피질순함량균수수온승고정선승후강，균재18℃체최대치；혈안함량칙축점하강，14℃시겁현저고우18℃、21℃시；새사Na＋?K＋?ATPase활력수수온승고이승고，단조간차이불현저。종상，봉폐순배수양식대릉평적괄의수온위16～18℃。
Four groups of juvenile turbots (371.68±43.15 g) were selected randomly to be reared at four constant water temperatures ( 14℃, 16℃, 18℃ and 21℃) , to examine the effect of the temperature on nitrogenous excretion, transaminase and serum immune factors of turbots in high stocking density ( 14. 20 ± 0.48 kg/m2 ) of closed recirculating aquaculture system for 56 days. The results indicated that: 1) The concentration of total ammonia nitrogen ( TAN) in water increased firstly and decreased as temperature increased, TAN of group 18℃was significantly higher than that of group 14 ℃and 21 ℃; nitrogenous excretion increased as temperature increased, described as an exponential function;2) The activities of GPT and GOT in liver increased firstly and decreased with increasing temperature. GPT and GOT of group 18℃ were highest. A reverse trend was found for GPT and GOT in serum, the maximum both occurred at group 21℃;3) The activities of LZM and SOD and Cortisol levels all increased firstly and decreased with increasing temperature, and the maximum all occurred at group 18℃. Ammonia levels decreased gradually as temperature increased, and the levels of group 14℃ were significantly higher than that of group 18 ℃ and 21℃. The activity of Na+?K+?ATPase increased gradually as temperature increased, but the differences among groups were not significant. It is concluded that the optimal water temperature of turbot in closed RAS was 16?18℃, providing the basis and reference for water temperature regulation of turbot in closed recirculating mariculture system.