CAJ | 학술논문

采用两对引物-聚合酶链反应（PCR-CTPP）方法分别对100例非小细胞肺癌患者和120例健康对照者进行p73基因多态性检测，DNA测序验证基因分型结果，对p73基因多态性与非小细胞肺癌的相关性进行分析，探讨湖南省地区人群中p73基因多态性与非小细胞肺癌遗传易感性的关系。试验结果表明，PCR-CTPP法和测序得到的基因分型结果完全吻合，100例肺癌患者的p73基因型分布如下：GC/GC型60例（60%）、GC/AT型36例（36%）、AT/AT型4例（4%），与携带p73 GC/GC基因型者比较，携带AT/AT基因型者的肺癌患病风险增加3.4倍（OR=3.387，95%CI=1.055~10.875，P=0.032），而携带GC/AT基因型者肺癌风险也呈增加趋势，但是无显著性差异（OR=1.183，95%CI=0.672~2.082，P=0.561）。p73基因多态性可能与湖南省地区人群非小细胞肺癌遗传易感性相关。
채용량대인물-취합매련반응（PCR-CTPP）방법분별대100례비소세포폐암환자화120례건강대조자진행p73기인다태성검측，DNA측서험증기인분형결과，대p73기인다태성여비소세포폐암적상관성진행분석，탐토호남성지구인군중p73기인다태성여비소세포폐암유전역감성적관계。시험결과표명，PCR-CTPP법화측서득도적기인분형결과완전문합，100례폐암환자적p73기인형분포여하：GC/GC형60례（60%）、GC/AT형36례（36%）、AT/AT형4례（4%），여휴대p73 GC/GC기인형자비교，휴대AT/AT기인형자적폐암환병풍험증가3.4배（OR=3.387，95%CI=1.055~10.875，P=0.032），이휴대GC/AT기인형자폐암풍험야정증가추세，단시무현저성차이（OR=1.183，95%CI=0.672~2.082，P=0.561）。p73기인다태성가능여호남성지구인군비소세포폐암유전역감성상관。
Used PCR-CTPP to detect the p73 gene polymorphism among 100 patients with NSCLC and 120 healthy controls respectively and DNA direct sequencing verified the results of genotypes. Analyzed the association between p73 gene polymorphism and NSCLC, and explored the correlation between p73 gene polymorphism and susceptibility to NSCLC in the Hunan population. The experiment results showed that the results of PCR-CTPP and the sequencing genotyping results were consistent, p73 genotype distribution in 100 lung cancer patients are as follows:GC/GC 60 cases (60%), GC/AT 36 cases (36%), AT/AT 4 cases (4%). Compared with the carriers of GC/GC genotype, the carriers of AT/AT genotype significantly increased 3.4 times the risk of NSCLC (OR=3.387, 95%CI=1.055~10.875, P=0.032), also the carriers of GC/AT genotype tended to increase the risk of NSCLC, but had no significantly difference (OR=1.183, 95%CI=0.672~2.082, P=0.561). The p73 gene polymorphism may have the relation with the susceptibility to NSCLC in the Hunan population.