广州医科大学学报
廣州醫科大學學報
엄주의과대학학보
Academic Journal of Guangzhou Medical College
2014年
4期
30-34
,共5页
糖尿病%血糖控制%低聚糖阿魏酸酯%脂质过氧化作用
糖尿病%血糖控製%低聚糖阿魏痠酯%脂質過氧化作用
당뇨병%혈당공제%저취당아위산지%지질과양화작용
diabetes%glycaemic control%feruloyl oligosaccharides%lipid peroxidation
目的：探讨低聚糖阿魏酸酯( CFOs)在由链脲佐菌素诱导的1型糖尿病怀孕大鼠体内的降血糖和抗氧化作用。方法：取40只大鼠做实验，共分五组(每组8只)：(1)未交配过的糖尿病鼠为对照组；(2)交配过的非糖尿病鼠为对照组；(3)交配过的糖尿病鼠为对照组；(4)未交配过的糖尿病鼠为治疗组；(5)交配过的糖尿病鼠为治疗组。治疗组在大鼠交配前3 d开始，连续15 d给大鼠喂饲玉米低聚糖阿魏酸酯植物提取物(25 mg/kg)。对照组动物则只接受溶媒处理(2.5 mL/kg)。在交配后的第14天，处死大鼠，从下腔静脉采集母体血液，做ELISA检测。交配过的大鼠应解剖其子宫以搜寻任何可能存在的胎儿。每只大鼠的肝脏都被迅速切除，用Jamall和Smith的检测方法来确定组织样本中脂质过氧化物的水平。结果：不考虑怀孕因素，低聚糖阿魏酸酯的使用可降低血糖值( P<0.05)。使用低聚糖阿魏酸酯治疗未孕和怀孕大鼠后，两者血清胰岛素水平均上升( P<0.05)。使用低聚糖阿魏酸酯并未对血浆内的硫代巴比妥酸反应物水平产生显著影响( P>0.05)。实验动物被诱导患糖尿病后，再用低聚糖阿魏酸酯进行治疗，其谷胱甘肽水平的平均值有所下降( P<0.05)。糖尿病孕鼠治疗组和孕鼠对照组肝脏内的硫代巴比妥酸反应物水平和谷胱甘肽水平，差异有统计学意义(P<0.05)。结论：低聚糖阿魏酸酯可降低怀孕及未怀孕糖尿病大鼠体内的血糖浓度、提高血清胰岛素浓度；与肝脏有关的脂质过氧化反应上升和谷胱甘肽的下降发生逆转。
目的：探討低聚糖阿魏痠酯( CFOs)在由鏈脲佐菌素誘導的1型糖尿病懷孕大鼠體內的降血糖和抗氧化作用。方法：取40隻大鼠做實驗，共分五組(每組8隻)：(1)未交配過的糖尿病鼠為對照組；(2)交配過的非糖尿病鼠為對照組；(3)交配過的糖尿病鼠為對照組；(4)未交配過的糖尿病鼠為治療組；(5)交配過的糖尿病鼠為治療組。治療組在大鼠交配前3 d開始，連續15 d給大鼠餵飼玉米低聚糖阿魏痠酯植物提取物(25 mg/kg)。對照組動物則隻接受溶媒處理(2.5 mL/kg)。在交配後的第14天，處死大鼠，從下腔靜脈採集母體血液，做ELISA檢測。交配過的大鼠應解剖其子宮以搜尋任何可能存在的胎兒。每隻大鼠的肝髒都被迅速切除，用Jamall和Smith的檢測方法來確定組織樣本中脂質過氧化物的水平。結果：不攷慮懷孕因素，低聚糖阿魏痠酯的使用可降低血糖值( P<0.05)。使用低聚糖阿魏痠酯治療未孕和懷孕大鼠後，兩者血清胰島素水平均上升( P<0.05)。使用低聚糖阿魏痠酯併未對血漿內的硫代巴比妥痠反應物水平產生顯著影響( P>0.05)。實驗動物被誘導患糖尿病後，再用低聚糖阿魏痠酯進行治療，其穀胱甘肽水平的平均值有所下降( P<0.05)。糖尿病孕鼠治療組和孕鼠對照組肝髒內的硫代巴比妥痠反應物水平和穀胱甘肽水平，差異有統計學意義(P<0.05)。結論：低聚糖阿魏痠酯可降低懷孕及未懷孕糖尿病大鼠體內的血糖濃度、提高血清胰島素濃度；與肝髒有關的脂質過氧化反應上升和穀胱甘肽的下降髮生逆轉。
목적：탐토저취당아위산지( CFOs)재유련뇨좌균소유도적1형당뇨병부잉대서체내적강혈당화항양화작용。방법：취40지대서주실험，공분오조(매조8지)：(1)미교배과적당뇨병서위대조조；(2)교배과적비당뇨병서위대조조；(3)교배과적당뇨병서위대조조；(4)미교배과적당뇨병서위치료조；(5)교배과적당뇨병서위치료조。치료조재대서교배전3 d개시，련속15 d급대서위사옥미저취당아위산지식물제취물(25 mg/kg)。대조조동물칙지접수용매처리(2.5 mL/kg)。재교배후적제14천，처사대서，종하강정맥채집모체혈액，주ELISA검측。교배과적대서응해부기자궁이수심임하가능존재적태인。매지대서적간장도피신속절제，용Jamall화Smith적검측방법래학정조직양본중지질과양화물적수평。결과：불고필부잉인소，저취당아위산지적사용가강저혈당치( P<0.05)。사용저취당아위산지치료미잉화부잉대서후，량자혈청이도소수평균상승( P<0.05)。사용저취당아위산지병미대혈장내적류대파비타산반응물수평산생현저영향( P>0.05)。실험동물피유도환당뇨병후，재용저취당아위산지진행치료，기곡광감태수평적평균치유소하강( P<0.05)。당뇨병잉서치료조화잉서대조조간장내적류대파비타산반응물수평화곡광감태수평，차이유통계학의의(P<0.05)。결론：저취당아위산지가강저부잉급미부잉당뇨병대서체내적혈당농도、제고혈청이도소농도；여간장유관적지질과양화반응상승화곡광감태적하강발생역전。
Objective: To investigate the hypoglycaemic and antioxidant effects of the corn feruloyl oligosaccharides ( CFOs ) in pregnant rat models with streptozotocin-induced type 1 diabetes. Methods: Five groups (n=8, each) were formed: (1) diabetic non-mated control group; (2) non-diabetic mated control group;( 3) diabetic mated control group; ( 4 ) diabetic non-mated treatment group and ( 5 ) diabetic mated treatment group. The CFOs plant extract ( 25 mg/kg) was administered orally for 15 continuous days in treatment groups, beginning 3 days before the rats mating. And the control groups only accepted menstruum treatment (2.5 mL/kg) . In the 14th day after mating, all rats were executed and the maternal venous blood was collected from inferior vena cava for ELISA detection. Mated rats should be dissected to search any possible fetuses in their uterus. The liver of each rat was quickly removed to determine the levels of lipid peroxide in tissue samples by detection method of Jamall and Smith. Results:Without the consideration of pregnant factors, the use of feruloyl oligosaccharides could decrease the value of blood glucose (P<0.05). After using feruloyl oligosaccharides to treat non-pregnant and pregnant rats, the levels of both serum insulin increased ( P<0. 05 ) . And the use of feruloyl oligosaccharides had no significant influence on the levels of thiobarbituric acid-reactive substances of the serum ( P>0.05) . After the experimental animals with induced diabetes treated by feruloyl oligosaccharides, their mean level of glutathione decreased (P<0.05). There were statistical significance of the levels of thiobarbituric acid-reactive substances and the levels of glutathione between the rat livers of diabetic pregnant treatment group and pregnant control group ( P<0.05) . Conclusion:Feruloyl oligosaccharides could decrease the concentration of blood glucose and increase the concentration of serum insulin in pregnant and non-pregnant diabetic rats. And the rise of lipid peroxidation and the decline of glutathione related with liver reversed.
