CAJ | 학술논문

目的：探讨体外诱导脐血产生特异性细胞毒淋巴细胞的可行性。方法：通过体外联合细胞因子体外诱导10份脐血单个核细胞分化为树突状细胞( dendritic cells,DCs),同时让DCs细胞负载U937冻融抗原；使成熟DCs刺激同源的脐血T淋巴细胞生成细胞毒性T细胞( cytotoxic T lymphocytes,CTLs)从而进行杀伤效应实验。 MTT法测定杀伤活性。结果：10份脐血标本均可培养出形态典型、功能成熟的DCs。经DCs诱导效应细胞DC-CTLs,在不同效靶比对U937细胞系、K562细胞株和对照组中,杀伤率以U937组最高( P<0.05)。结论：特定抗原负载的脐血DCs细胞有特异性的杀伤效应。
목적：탐토체외유도제혈산생특이성세포독림파세포적가행성。방법：통과체외연합세포인자체외유도10빈제혈단개핵세포분화위수돌상세포( dendritic cells,DCs),동시양DCs세포부재U937동융항원；사성숙DCs자격동원적제혈T림파세포생성세포독성T세포( cytotoxic T lymphocytes,CTLs)종이진행살상효응실험。 MTT법측정살상활성。결과：10빈제혈표본균가배양출형태전형、공능성숙적DCs。경DCs유도효응세포DC-CTLs,재불동효파비대U937세포계、K562세포주화대조조중,살상솔이U937조최고( P<0.05)。결론：특정항원부재적제혈DCs세포유특이성적살상효응。
Objective:To investigate the feasibility of inducing specific cytotoxic T lymphocytes by cord blood in vitro. Methods:Mononuclear cells from 10 induced samples of cord blood were differentiated into dendritic cells ( DCs) by cytokines in vitro, and DCs cells were loading with freeze-thaw antigen of U937 at the same time. Homologous cord blood T lymphocytes activated by mature cells generated cytotoxic T lymphocytes ( CLTs) for the experiment of lethal effect. The killing activity was assayed with MTT method. Results: Mature DCs with typical morphology and function were cultivated from 10 samples of cord blood. As effector cell DC-CTLs were induced through DCs, the target ratio was compared amongU937 cell line group, K562 cell line group and the control group. It showed that the highest killing rate was in U937 group (P<0.05). Conclusion: DCs cells of cord blood loaded with specific antigen have specific killing effect.